Linked Life Data

17529926

Source:http://linkedlifedata.com/resource/pubmed/id/17529926

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-6-14
pubmed:abstractText
Barrett's esophagus is lined by columnar and goblets cells with gastric and intestinal characteristics. Despite the association between goblet elements and malignancy, it was not demonstrated that other columnar cells lineages are not related to neoplasia. Chromosomal abnormalities were described in metaplasia adjacent to Barrett's neoplasia, but it is unknown which metaplastic lineages are involved. This work assessed the frequency and the type of chromosomal abnormalities in Barrett's esophagus without neoplasia and performed the identification of the metaplastic cells carrying chromosomal gains. Barrett's esophagus biopsies were collected and processed for short-term cell culture and cytogenetic analysis. Combined immunofluorescence/fluorescence in situ hybridization was performed in cases exhibiting chromosomal gains by using antisera against intestinal (MUC2) and gastric (MUC5AC and MUC6) apomucins and chromosome pericentromeric alpha satellite DNA probes for the chromosomes involved. Each case was scored for the number of spots (0, 1, 2, >2) in 200 nonoverlapping nuclei. Columnar and goblet cells were separately assessed. Short-term cell cultures were achieved in 40/60 cases (67%). There were clonal abnormalities in 27/40 cases (68%) and tetraploid (4n) clones in 10/40 (25%). Structural alterations were detected in 14/40 (35%) with recurrent breakpoints at 1q21, 15q15 and 15q22. Numerical changes (trisomies 7 and 18 and loss of Y) occurred in 16/40 (40%). Gains of chromosomes 7 and 18 were more frequent in columnar than in goblet cells (9.8% vs 0.7% (P<0.05)) and (7.9 vs 1.9% (P<0.05)) respectively. These alterations were detected in cells exhibiting gastric as well as intestinal features and were more frequent in cells without apomucin production. Conclusions: (1) chromosomal instability is a common finding in Barrett's esophagus without neoplasia. (2) The two metaplastic populations are committed, chromosomal gains being more frequent in columnar nongoblet than in goblet cells. (3) The two metaplastic phenotypes, gastric and intestinal, are equally involved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0893-3952
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
788-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17529926-Adult, pubmed-meshheading:17529926-Aged, pubmed-meshheading:17529926-Aged, 80 and over, pubmed-meshheading:17529926-Barrett Esophagus, pubmed-meshheading:17529926-Biopsy, pubmed-meshheading:17529926-Cells, Cultured, pubmed-meshheading:17529926-Chromosomal Instability, pubmed-meshheading:17529926-Chromosome Aberrations, pubmed-meshheading:17529926-Chromosomes, Human, Pair 18, pubmed-meshheading:17529926-Chromosomes, Human, Pair 7, pubmed-meshheading:17529926-Esophagus, pubmed-meshheading:17529926-Female, pubmed-meshheading:17529926-Goblet Cells, pubmed-meshheading:17529926-Humans, pubmed-meshheading:17529926-In Situ Hybridization, Fluorescence, pubmed-meshheading:17529926-Karyotyping, pubmed-meshheading:17529926-Male, pubmed-meshheading:17529926-Metaphase, pubmed-meshheading:17529926-Middle Aged, pubmed-meshheading:17529926-Mucin 5AC, pubmed-meshheading:17529926-Mucin-2, pubmed-meshheading:17529926-Mucin-6, pubmed-meshheading:17529926-Mucins
pubmed:year
2007
pubmed:articleTitle
Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved.
pubmed:affiliation
Grupo de Estudo do Esófago de Barrett, Instituto Português de Oncologia de Lisboa de Francisco Gentil, EPE, Lisboa, Portugal. pchaves@ipolisboa.min-saude.pt
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't