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rdf:type |
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lifeskim:mentions |
umls-concept:C0027882,
umls-concept:C0030685,
umls-concept:C0183210,
umls-concept:C0391871,
umls-concept:C0456962,
umls-concept:C0680255,
umls-concept:C0871161,
umls-concept:C1283071,
umls-concept:C1515021,
umls-concept:C1521902,
umls-concept:C1963578
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pubmed:issue |
4
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pubmed:dateCreated |
2007-5-24
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pubmed:abstractText |
Synaptotagmin-1 and -2 are known Ca(2+) sensors for fast synchronous neurotransmitter release, but the potential Ca(2+)-sensor functions of other synaptotagmins in release remain uncharacterized. We now show that besides synaptotagmin-1 and -2, only synaptotagmin-9 (also called synaptotagmin-5) mediates fast Ca(2+) triggering of release. Release induced by the three different synaptotagmin Ca(2+) sensors exhibits distinct kinetics and apparent Ca(2+) sensitivities, suggesting that the synaptotagmin isoform expressed by a neuron determines the release properties of its synapses. Conditional knockout mice producing GFP-tagged synaptotagmin-9 revealed that synaptotagmin-9 is primarily expressed in the limbic system and striatum. Acute deletion of synaptotagmin-9 in striatal neurons severely impaired fast synchronous release without changing the size of the readily-releasable vesicle pool. These data show that in mammalian brain, only synaptotagmin-1, -2, and -9 function as Ca(2+) sensors for fast release, and that these synaptotagmins are differentially expressed to confer distinct release properties onto synapses formed by defined subsets of neurons.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
567-81
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pubmed:meshHeading |
pubmed-meshheading:17521570-Animals,
pubmed-meshheading:17521570-Animals, Newborn,
pubmed-meshheading:17521570-Calcium,
pubmed-meshheading:17521570-Cells, Cultured,
pubmed-meshheading:17521570-Corpus Striatum,
pubmed-meshheading:17521570-Gene Expression Regulation,
pubmed-meshheading:17521570-Green Fluorescent Proteins,
pubmed-meshheading:17521570-Inhibitory Postsynaptic Potentials,
pubmed-meshheading:17521570-Membrane Potentials,
pubmed-meshheading:17521570-Mice,
pubmed-meshheading:17521570-Mice, Transgenic,
pubmed-meshheading:17521570-Microtubule-Associated Proteins,
pubmed-meshheading:17521570-Neurons,
pubmed-meshheading:17521570-Patch-Clamp Techniques,
pubmed-meshheading:17521570-Presynaptic Terminals,
pubmed-meshheading:17521570-Synapsins,
pubmed-meshheading:17521570-Synaptotagmin I,
pubmed-meshheading:17521570-Synaptotagmin II,
pubmed-meshheading:17521570-Synaptotagmins
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pubmed:year |
2007
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pubmed:articleTitle |
Synaptotagmin-1, -2, and -9: Ca(2+) sensors for fast release that specify distinct presynaptic properties in subsets of neurons.
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pubmed:affiliation |
Department of Neuroscience, The University of Texas Southwestern Medical Center, Dallas TX 75390, USA.
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pubmed:publicationType |
Journal Article
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