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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2007-6-7
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pubmed:abstractText |
The spliced leader (SL) RNA provides the 5' m7G cap and first 39 nt for all nuclear mRNAs in kinetoplastids. This small nuclear RNA is transcribed by RNA polymerase II from individual promoters. In Leishmania tarentolae the SL RNA genes reside in two multi-copy tandem arrays designated MINA and MINB. The transcript accumulation from the SL promoter on the drug-selected, episomal SL RNA gene cassette pX-tSL is ~10% that of the genomic array in uncloned L. tarentolae transfectants. This disparity is neither sequence- nor copy-number related, and thus may be due to interference of SL promoter function by epigenetic factors. To explore these possibilities we examined the nucleoplasmic localization of the SL RNA genes as well as their nucleosomal architecture.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2180
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17517143-Animals,
pubmed-meshheading:17517143-Blotting, Southern,
pubmed-meshheading:17517143-Cell Nucleus,
pubmed-meshheading:17517143-In Situ Hybridization, Fluorescence,
pubmed-meshheading:17517143-Leishmania,
pubmed-meshheading:17517143-Nucleosomes,
pubmed-meshheading:17517143-Promoter Regions, Genetic,
pubmed-meshheading:17517143-RNA, Spliced Leader,
pubmed-meshheading:17517143-Transcription, Genetic
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pubmed:year |
2007
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pubmed:articleTitle |
The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes.
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pubmed:affiliation |
Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095-1489, USA. mrbob@ucla.edu <mrbob@ucla.edu>
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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