Linked Life Data

17516161

Source:http://linkedlifedata.com/resource/pubmed/id/17516161

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-7-19
pubmed:databankReference
pubmed:abstractText
Marek's disease (MD) is a highly contagious lymphoproliferative disease of chickens. Meq is the relevant oncogene and four isoforms, long (L)-meq, meq, short (S)-meq and very short (VS)-meq, have been identified. Although MD is important in the poultry industry, the prevalence and molecular properties of Korean MD virus (MDV) among broiler chickens remain unclear. Therefore, we characterized meq in pooled feather tips sampled at 3- and 5-week-old chickens from 21 unvaccinated and 22 vaccinated broiler farms via nested-PCR and nucleotide sequence analysis. Multiple bands consisting of L-meq, meq, and S-meq amplicons were observed in a commercial vaccine (CVI988 + HVT), 1 (4.8%) and 5 samples (22.7%) from unvaccinated and vaccinated farms, respectively. A strong meq amplicon was observed in a MD-related tumor tissue, 6 (28.6%) and 1 (4.5%) samples from unvaccinated and vaccinated farms, respectively. Six and one amplicons from unvaccinated (28.6%) and vaccinated farms (4.5%), respectively, were differentiated from CVI988 by nucleotide sequence analysis. Therefore, the relatively high rate of meq in the unvaccinated broiler farms constitutes support for vaccination. However, the existence of CVI988-related meq in unvaccinated chickens necessitates further study regarding the origins and pathoimmunological effects of the viruses on chickens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0920-8569
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-45
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Prevalence and molecular characterization of meq in feather follicular epithelial cells of Korean broiler chickens.
pubmed:affiliation
Department of Microbiology, Seoul National University, Seoul 151-742, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't