Linked Life Data

17513458

Source:http://linkedlifedata.com/resource/pubmed/id/17513458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-8-1
pubmed:abstractText
The fetal lung normally contains immature monocytes and very few mature macrophages. The chorioamnionitis frequently associated with preterm birth induces monocyte influx into the fetal lung. Previous studies demonstrated that monocytes in the developing lung can mediate lung injury responses that resemble BPD in humans. We hypothesized that chorioamnionitis would induce maturation of immature monocytes in the fetal lung. Groups of three to seven time-mated ewes received saline or 10 mg of endotoxin (Escherichia coli 055:B5) in saline by intra-amniotic injection for intervals from 1 to 14 days before operative delivery at 124 days of gestational age. Monocytic cells from lung tissue were recovered using Percoll gradients. Monocytic cells consistent with macrophages were identified morphologically and by myosin heavy chain class II expression. An increase in macrophages was preceded by induction of granulocyte-macrophage colony-stimulating factor in the lung and subsequent activation of the transcription factor PU.1. The production of IL-6 by monocytes/macrophages in response to endotoxin challenge in vitro increased 7 and 14 days after exposure to intra-amniotic endotoxin. Recombinant TNF-alpha induced IL-6 production by lung monocytic cells exposed to intra-amniotic endotoxin but not in control cells. Monocytic phagocytosis of apoptotic neutrophils also increased 7 and 14 days after exposure to intra-amniotic endotoxin. Intra-amniotic endotoxin induced lung monocytes to develop into functionally mature cells consistent with macrophages. These findings have implications for lung immune responses after exposure to chorioamnionitis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1040-0605
pubmed:author
pubmed:issnType
Print
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L345-53
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17513458-Animals, pubmed-meshheading:17513458-Animals, Newborn, pubmed-meshheading:17513458-Apoptosis, pubmed-meshheading:17513458-Chorioamnionitis, pubmed-meshheading:17513458-Endotoxins, pubmed-meshheading:17513458-Female, pubmed-meshheading:17513458-Gestational Age, pubmed-meshheading:17513458-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:17513458-Immunophenotyping, pubmed-meshheading:17513458-Interleukin-6, pubmed-meshheading:17513458-Lung, pubmed-meshheading:17513458-Macrophages, pubmed-meshheading:17513458-Monocytes, pubmed-meshheading:17513458-Neutrophils, pubmed-meshheading:17513458-Phagocytosis, pubmed-meshheading:17513458-Pregnancy, pubmed-meshheading:17513458-Proto-Oncogene Proteins, pubmed-meshheading:17513458-RNA, Messenger, pubmed-meshheading:17513458-Recombinant Proteins, pubmed-meshheading:17513458-Sheep, pubmed-meshheading:17513458-Trans-Activators, pubmed-meshheading:17513458-Tumor Necrosis Factor-alpha
pubmed:year
2007
pubmed:articleTitle
Endotoxin-induced maturation of monocytes in preterm fetal sheep lung.
pubmed:affiliation
University Hospital Maastricht, Maastricht, The Netherlands. bkra@paed.azm.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural