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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-5-29
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pubmed:abstractText |
Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. Our previous studies also revealed that STAP-2 binds to MyD88 and IKK-alpha/beta, and modulates NF-kappaB signaling in macrophages. In the present study, we examined physiological roles of the interaction between STAP-2 and c-Fms in Raw 264.7 macrophage cells. Our immunoprecipitation has revealed that c-Fms directly interacts with the PH domain of STAP-2 independently on M-CSF-stimulation. Ectopic expression of STAP-2 markedly suppressed M-CSF-induced tyrosine phosphorylation of c-Fms as well as activation of Akt and extracellular signal regulated kinase. In addition, Raw 264.7 cells over-expressing STAP-2 showed impaired migration in response to M-CSF and wound-healing process. Taken together, our findings demonstrate that STAP-2 directly binds to c-Fms and interferes with the PI3K signaling, which leads to macrophage motility, in Raw 264.7 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Endosomal Sorting Complexes...,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Stam2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
358
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
931-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:17512498-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:17512498-Animals,
pubmed-meshheading:17512498-Cell Line,
pubmed-meshheading:17512498-Cell Movement,
pubmed-meshheading:17512498-Endosomal Sorting Complexes Required for Transport,
pubmed-meshheading:17512498-Gene Expression Regulation,
pubmed-meshheading:17512498-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:17512498-Macrophages,
pubmed-meshheading:17512498-Mice,
pubmed-meshheading:17512498-Models, Biological,
pubmed-meshheading:17512498-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:17512498-Phosphoproteins,
pubmed-meshheading:17512498-Phosphorylation,
pubmed-meshheading:17512498-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:17512498-Signal Transduction,
pubmed-meshheading:17512498-Tyrosine,
pubmed-meshheading:17512498-Wound Healing
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pubmed:year |
2007
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pubmed:articleTitle |
STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.
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pubmed:affiliation |
Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo 060-0812, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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