Source:http://linkedlifedata.com/resource/pubmed/id/17508907
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2007-5-22
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| pubmed:abstractText |
The effects of nuclear-localized oxidative stress on both nuclear antioxidant systems, and the processes that they regulate, are not clearly understood. Here, we targeted a hydrogen peroxide (H(2)O(2))-producing enzyme, D-amino acid oxidase (DAAO), to the nucleus (NLS-DAAO) and used this to generate H(2)O(2) in the nuclei of cells. On addition of N-acetyl-D-alanine (NADA), a substrate of DAAO, to NLS-DAAO-transfected HeLa cells, a twofold increase in ROS production relative to untreated, transfected control was observed. Staining of cellular thiols confirmed that NLS-DAAO-induced ROS selectively modified the nuclear thiol pool, whereas the cytoplasmic pool remained unchanged. Furthermore, NLS-DAAO/NADA-induced ROS caused significant oxidation of the nuclear GSH pool, as measured by nuclear protein S-glutathionylation (Pr-SSG), but under the same conditions, nuclear Trx1 redox state was not altered significantly. NF-kappaB reporter activity was diminished by NLS-DAAO/NADA-stimulated nuclear oxidation. We conclude that nuclear GSH is more susceptible to localized oxidation than is nuclear Trx1. Furthermore, the attenuation of NF-kappaB reporter activity in the absence of nuclear Trx1 oxidation suggests that critical nuclear redox proteins are subject to control by S-glutathionylation during oxidative stress in the nucleus.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/D-Amino-Acid Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Disulfide,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Thioredoxins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1523-0864
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
9
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
807-16
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:17508907-Active Transport, Cell Nucleus,
pubmed-meshheading:17508907-Alanine,
pubmed-meshheading:17508907-Blotting, Western,
pubmed-meshheading:17508907-Cell Nucleus,
pubmed-meshheading:17508907-Cell Survival,
pubmed-meshheading:17508907-D-Amino-Acid Oxidase,
pubmed-meshheading:17508907-Glutathione,
pubmed-meshheading:17508907-Glutathione Disulfide,
pubmed-meshheading:17508907-HeLa Cells,
pubmed-meshheading:17508907-Humans,
pubmed-meshheading:17508907-Hydrogen Peroxide,
pubmed-meshheading:17508907-Immunoprecipitation,
pubmed-meshheading:17508907-Models, Biological,
pubmed-meshheading:17508907-NF-kappa B,
pubmed-meshheading:17508907-Oxidation-Reduction,
pubmed-meshheading:17508907-Oxidative Stress,
pubmed-meshheading:17508907-Reactive Oxygen Species,
pubmed-meshheading:17508907-Thioredoxins,
pubmed-meshheading:17508907-Transfection
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| pubmed:year |
2007
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| pubmed:articleTitle |
Selective oxidative stress in cell nuclei by nuclear-targeted D-amino acid oxidase.
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| pubmed:affiliation |
Division of Pulmonary, Allergy, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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