Linked Life Data

17504564

Source:http://linkedlifedata.com/resource/pubmed/id/17504564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-5-16
pubmed:abstractText
A simplified and efficient method is developed for the large-scale purification of the secoisolariciresinol diglucoside (SDG) from defatted flaxseed after aqueous ethanol extraction. Extractant from defatted flaxseed with aqueous ethanol is hydrolyzed with basic solution, concentrated under vacuum, subjected to Sephadex LH-20 column chromatography, and eluted with aqueous ethanol of different concentrations. Elution is monitored by a UV detector at 280 nm, and fractions containing SDG are pooled, concentrated, and applied to a second column chromatography under the same conditions. Elution with water results in a better resolution of SDG [94.5% by high-performance liquid chromatography (HPLC)] than that with pure ethanol or 50% (v/v) aqueous ethanol. HPLC-photodiode array detection-mass spectrometry and NMR are applied to identify SDG and to determine the purity of the eluted fraction. This simplified purification scheme avoids toxic organic solvent used in the common silica gel separation process and, thus, increases the safety of the process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9665
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-82
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Purification of secoisolariciresinol diglucoside with column chromatography on a sephadex LH-20.
pubmed:affiliation
School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, PR China.
pubmed:publicationType
Journal Article