Linked Life Data

17492833

Source:http://linkedlifedata.com/resource/pubmed/id/17492833

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-6-1
pubmed:abstractText
The accomplishment of the quantification of the recombinant protein content of whole bacterial cells by FT-IR spectroscopy by application of chemometrics is shown. Recombinant Escherichia coli cells expressing an inclusion body forming fusion protein were dried on a 96-well silicon plate for the analysis in a high-throughput FT-IR spectrometer. Acquired spectra of additionally conventionally quantified samples were used to establish a multivariate calibration. The obtained method was tested by predicting inclusion body contents of samples not used for the multivariate model. Results from FT-IR spectra coincided well with the data of universalized electrophoresis analysis. Hence FT-IR spectroscopy could prove as a fast and simple alternative to conventional quantification methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
8756-7938
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
762-6
pubmed:meshHeading
pubmed:articleTitle
Fast quantification of recombinant protein inclusion bodies within intact cells by FT-IR spectroscopy.
pubmed:affiliation
Department of Biotechnology, University of Natural Resources, Applied Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't