| pubmed-article:17491147 | pubmed:abstractText | The two types of cells that make up the cumulus-oocyte complex (i.e. the oocyte and cumulus cells) have very different metabolic demands, with glucose occupying a central role in metabolic activity. Cumulus cells have a significant requirement for and utilise high levels of glucose, yet appear to have little need for oxidative metabolism. In contrast, oocytes have a requirement for oxidative metabolism, although limited glucose metabolism may also be an important aspect of meiotic and developmental competence. Nevertheless, because of the metabolic and communication link between the cumulus and the oocyte, glucose availability and metabolism within the cumulus can have a significant impact on oocyte meiotic and developmental competence. In particular, the role of the hexosamine biosynthesis pathway within cumulus cells appears critical for the supply of substrate from glucose for extracellular matrix production, yet if overstimulated can significantly decrease developmental competence of the oocyte. Current static systems for in vitro maturation are clearly incompatible with meeting substrate demands, especially glucose. In the future, in vitro maturation will include a more dynamic approach, which will adjust nutrient components to meet the changing functional requirements of cumulus-oocyte complexes during the final process of maturation. | lld:pubmed |
