|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2007-5-10
|
|
pubmed:abstractText |
The study was purposed to explore the effects of NKG2D receptor and its ligands RAE-1 and H60 on graft-versus-tumor (GVT) response induced by MHC haploidentical bone marrow/spleen cell transplantation. Female (BALB/c x C57BL/6) F1 mice (CB6F1, H-2K(b/d)) inoculated with H22 cells to develop a solid tumor model were the recipients, and bone marrow mixed with spleen cells of the healthy male C57BL/6 (H-2K(b)) mice were the donor cells. GVT response was observed after transplantation that from donor cells T and NK cells were purged with anti-CD3 and anti-NK monoclonal antibody, and the NKG2D receptor was blocked with anti-NKG2D monoclonal antibody, the expression levels of RAE-1 and H60 mRNA in tumor tissue were measured by means of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points after transplantation. The results showed that the GVT response of transplantation was reduced after in vitro depletion of T and NK cells or blocking NKG2D receptor in donor cells of the graft, the expression levels of RAE-1 and H60 mRNA in tumor tissue increased after transplantation of haploidential bone marrow mixed with spleen cells. It is concluded that NKG2D and its ligands RAE-1 and H60 may play important roles in GVT response.
|
|
pubmed:language |
chi
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Klrk1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Minor Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Matrix-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocytoplasmic Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rae1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Natural Killer Cell,
http://linkedlifedata.com/resource/pubmed/chemical/minor H antigen H60
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
1009-2137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
15
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
160-4
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:17490545-Animals,
pubmed-meshheading:17490545-Female,
pubmed-meshheading:17490545-Graft vs Leukemia Effect,
pubmed-meshheading:17490545-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:17490545-Killer Cells, Natural,
pubmed-meshheading:17490545-Leukemia, Experimental,
pubmed-meshheading:17490545-Ligands,
pubmed-meshheading:17490545-Male,
pubmed-meshheading:17490545-Mice,
pubmed-meshheading:17490545-Mice, Inbred BALB C,
pubmed-meshheading:17490545-Mice, Inbred C57BL,
pubmed-meshheading:17490545-Mice, Inbred Strains,
pubmed-meshheading:17490545-Minor Histocompatibility Antigens,
pubmed-meshheading:17490545-NK Cell Lectin-Like Receptor Subfamily K,
pubmed-meshheading:17490545-Nuclear Matrix-Associated Proteins,
pubmed-meshheading:17490545-Nucleocytoplasmic Transport Proteins,
pubmed-meshheading:17490545-Receptors, Immunologic,
pubmed-meshheading:17490545-Receptors, Natural Killer Cell
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
[Effects of NKG2D and its ligands RAE-1 and H60 on graft-versus-tumor response].
|
|
pubmed:affiliation |
Department of Oncology, Quanzhou Hospital for Traditional Chinese Medicine, Quanzhou 362000, China.
|
|
pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
|
