rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
7
|
pubmed:dateCreated |
2007-7-2
|
pubmed:abstractText |
RNA interference (RNAi) is a powerful and widely used gene silencing strategy for studying gene function in mammalian cells. Transient or constitutive expression of either small interfering RNA (siRNA) or short hairpin RNA (shRNA) results in temporal or persistent inhibition of gene expression, respectively. A tightly regulated and reversibly inducible RNAi-mediated gene silencing approach could conditionally control gene expression in a temporal or spatial manner that provides an extremely useful tool for studying gene function involved in cell growth, survival and development.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
1099-498X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
9
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
620-34
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:17486668-Animals,
pubmed-meshheading:17486668-Cell Line,
pubmed-meshheading:17486668-Cricetinae,
pubmed-meshheading:17486668-Gene Expression,
pubmed-meshheading:17486668-Genetic Vectors,
pubmed-meshheading:17486668-HeLa Cells,
pubmed-meshheading:17486668-Humans,
pubmed-meshheading:17486668-Isopropyl Thiogalactoside,
pubmed-meshheading:17486668-Luciferases,
pubmed-meshheading:17486668-Operator Regions, Genetic,
pubmed-meshheading:17486668-Promoter Regions, Genetic,
pubmed-meshheading:17486668-RNA, Small Interfering,
pubmed-meshheading:17486668-RNA Interference,
pubmed-meshheading:17486668-Repressor Proteins,
pubmed-meshheading:17486668-Transfection,
pubmed-meshheading:17486668-Tumor Suppressor Protein p53
|
pubmed:year |
2007
|
pubmed:articleTitle |
A tightly regulated and reversibly inducible siRNA expression system for conditional RNAi-mediated gene silencing in mammalian cells.
|
pubmed:affiliation |
Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan 701, Taiwan, ROC.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|