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rdf:type |
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lifeskim:mentions |
umls-concept:C0009780,
umls-concept:C0012984,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0185117,
umls-concept:C0205360,
umls-concept:C0521339,
umls-concept:C1514811,
umls-concept:C1561577,
umls-concept:C1706462,
umls-concept:C2911684
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pubmed:dateCreated |
2007-5-31
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pubmed:abstractText |
The quantification of gene expression in tissue samples requires the use of reference genes to normalise transcript numbers between different samples. Reference gene stability may vary between different tissues, and between the same tissue in different disease states. We evaluated the stability of 9 reference genes commonly used in human gene expression studies. Real-time reverse transcription PCR and a mathematical algorithm were used to establish which reference genes were most stably expressed in normal and diseased canine articular tissues and two canine cell lines stimulated with lipolysaccaride (LPS).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-10772538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-10889296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-11762938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12082286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12137780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12184808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12200227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12464555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12525378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12757132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-12840047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-14647279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15127793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15283208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15289330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15331581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15334463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15519565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15702688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15705200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15757961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15800207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-15815687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-16324220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-16771605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-16844072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-16881859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-16984641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-17032449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-17099150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-1727936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-3350786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-4726075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-7337280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-8578897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-9187757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-9291440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17484782-9747782
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
1746-6148
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17484782-Adipose Tissue,
pubmed-meshheading:17484782-Algorithms,
pubmed-meshheading:17484782-Animals,
pubmed-meshheading:17484782-Cartilage, Articular,
pubmed-meshheading:17484782-Cell Line,
pubmed-meshheading:17484782-Dog Diseases,
pubmed-meshheading:17484782-Dogs,
pubmed-meshheading:17484782-Gene Expression Regulation,
pubmed-meshheading:17484782-Hindlimb,
pubmed-meshheading:17484782-Joints,
pubmed-meshheading:17484782-Osteoarthritis,
pubmed-meshheading:17484782-Reference Standards,
pubmed-meshheading:17484782-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:year |
2007
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pubmed:articleTitle |
Expression stability of commonly used reference genes in canine articular connective tissues.
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pubmed:affiliation |
Centre for Integrated Genomic Medical Research, The Manchester Interdisciplinary Biocentre, University of Manchester, UK. Duncan.Ayers-2@postgrad.manchester.ac.uk
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pubmed:publicationType |
Journal Article
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