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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2007-5-9
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pubmed:abstractText |
To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-10085368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-10862703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-11086982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-11779476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-12060707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-12540832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-12565839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-12684483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-14615590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-15131343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-15190253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-15558062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-15820685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16116447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16298005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16306259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16360690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16408092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16600853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16906137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-16950113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-2999347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-7689225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17483470-9125370
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:AugustineG JGJ,
pubmed-author:BoydenEE,
pubmed-author:DeisserothKK,
pubmed-author:FendJJ,
pubmed-author:HallW CWC,
pubmed-author:KasaiHH,
pubmed-author:MatsuzakiKK,
pubmed-author:MatsuzakiMM,
pubmed-author:NoguchiJJ,
pubmed-author:PeckDD,
pubmed-author:QiuLL,
pubmed-author:WangDD,
pubmed-author:WangHH,
pubmed-author:ZhangFF
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8143-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2007
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pubmed:articleTitle |
High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice.
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pubmed:affiliation |
Department of Neurobiology, Duke University, Durham, NC 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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