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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1992-1-22
|
| pubmed:abstractText |
Activation of the ras oncogene was investigated in esophageal tumors induced by methylbenzylnitrosamine (MBN) in the Sprague-Dawley rat. DNA was extracted from grossly visible carcinogen-induced tumors. H-ras and K-ras gene sequences were then amplified by the polymerase chain reaction. Point mutations in the ras genes were then identified by selective hybridization to allele-specific oligonucleotide probes. A guanine to adenine transition at the 35th nucleotide in the H-ras coding sequence (GGA to GAA in the 12 codon) was observed in 67% (10 of 15) of the papillomas examined. This mutation codes for glutamate instead of glycine as the 12th amino acid of the ras p21 protein. No other H-ras or K-ras mutations were observed. To determine the distribution of this H-ras mutation in esophageal tissues, histological sections of MBN-treated esophagi were stained with a monoclonal antibody (E184) which selectively recognizes the mutated ras p-21 with glutamate substituted for glycine as the 12th amino acid. Expression of the mutant ras p21 protein was observed in 20% of the squamous papillomas, 13.6% of hyperplastic lesions and 10% of dysplastic lesions. Thus, activation of the H-ras oncogene as a result of guanine to adenine point mutation is a frequent event in esophageal tumors induced by MBN, occurring in 67% of squamous papillomas, but expression of the corresponding mutant ras p21 protein is observed in a much smaller proportion of the tumors in this animal model.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethylnitrosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p21(ras),
http://linkedlifedata.com/resource/pubmed/chemical/nitrosobenzylmethylamine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0143-3334
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:geneSymbol |
H-ras,
ras
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2373-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1747941-Adenine,
pubmed-meshheading:1747941-Animals,
pubmed-meshheading:1747941-Base Sequence,
pubmed-meshheading:1747941-Carcinogens,
pubmed-meshheading:1747941-Codon,
pubmed-meshheading:1747941-Dimethylnitrosamine,
pubmed-meshheading:1747941-Esophageal Neoplasms,
pubmed-meshheading:1747941-Esophagus,
pubmed-meshheading:1747941-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:1747941-Genes, ras,
pubmed-meshheading:1747941-Guanine,
pubmed-meshheading:1747941-Immunohistochemistry,
pubmed-meshheading:1747941-Male,
pubmed-meshheading:1747941-Molecular Sequence Data,
pubmed-meshheading:1747941-Mutation,
pubmed-meshheading:1747941-Oncogene Protein p21(ras),
pubmed-meshheading:1747941-Papilloma,
pubmed-meshheading:1747941-Rats,
pubmed-meshheading:1747941-Rats, Inbred Strains
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Incidence of Harvey ras oncogene point mutations and their expression in methylbenzylnitrosamine-induced esophageal tumorigenesis.
|
| pubmed:affiliation |
Department of Medicine, Northwestern University Medical School, Chicago, IL.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|