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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-1-15
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pubmed:abstractText |
The effects of pertussis toxin on the Na(+)-dependent transport of uridine were studied in HL-60 leukaemia cells induced to differentiate along the granulocytic or monocytic pathways by dimethyl sulphoxide (DMSO) or phorbol 12-myristate 13-acetate (PMA) respectively. Pertussis toxin at 50 ng/ml completely inhibited the activation of Na(+)-dependent uridine transport and consequently prevented the formation of intracellular pools of free uridine which occurs in HL-60 cells induced to differentiate by DMSO. The inhibition of Na(+)-dependent uridine transport by pertussis toxin in cells exposed to DMSO was associated with a 14-fold decrease in affinity, with no change in Vmax. Pertussis toxin, however, had no effect on Na(+)-dependent uridine transport in PMA-induced HL-60 cells. Furthermore, 500 ng of cholera toxin/ml had no effect on the Na(+)-dependent uptake of uridine in DMSO-treated HL-60 cells. These results suggest that the activation of the Na(+)-dependent transport of uridine in HL-60 cells induced to differentiate along the granulocytic pathway by DMSO is coupled to a pertussis-toxin-sensitive guanine-nucleotide binding protein (G-protein).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-139409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2001243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2001255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2337150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2419757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2436299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2556387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2870796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2917162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2930492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-2971045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3003581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3006254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3020540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3025447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3106040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3110624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3113327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-3315030,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-4034370,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-6347269,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1747127-818136
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
280 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
515-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1747127-Biological Transport,
pubmed-meshheading:1747127-Cell Differentiation,
pubmed-meshheading:1747127-Dimethyl Sulfoxide,
pubmed-meshheading:1747127-Leukemia, Experimental,
pubmed-meshheading:1747127-Pertussis Toxin,
pubmed-meshheading:1747127-Sodium,
pubmed-meshheading:1747127-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1747127-Tumor Cells, Cultured,
pubmed-meshheading:1747127-Uridine,
pubmed-meshheading:1747127-Virulence Factors, Bordetella
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pubmed:year |
1991
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pubmed:articleTitle |
Inhibition by pertussis toxin of the activation of Na(+)-dependent uridine transport in dimethyl-sulphoxide-induced HL-60 leukaemia cells.
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pubmed:affiliation |
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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