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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1992-1-14
|
| pubmed:abstractText |
Epithelial cells from the cervical loop of the rat incisor were isolated by co-culture of apical explants with growth-arrested 3T3 fibroblasts. The epithelial phenotype of the expanding outgrowths was confirmed 10 days after the seeding of the explants by phase-contrast microscopy and immunocytochemical identification of cytokeratins. After 3 weeks in culture, the epithelial cells covered the entire surface of the coverslips and were then passaged. Subcultures gave rise to a confluent sheet within 10-12 days. Light and electron microscopy showed that confluent cervical epithelial cells generally reconstituted a bi-layered structure similar to Hertwig's epithelial sheath. Epithelial cells from the rat palate, cultured and subcultured according to the same procedure, organized themselves in 5-6 cell layers, the upper cells having generally a squamous morphology. Synthesis of extracellular matrix molecules by rat incisor cervical epithelial cells was studied with specific antibodies. These cells failed to produce type I collagen, but synthesized all the major basement membrane components (type IV collagen, laminin, heparan sulphate proteoglycan and fibronectin). These observations suggest that the culture conditions allowed the reconstitution of a typical Hertwig's epithelial sheath by rat incisor cervical epithelial cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0003-9969
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
737-45
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1747074-Animals,
pubmed-meshheading:1747074-Cells, Cultured,
pubmed-meshheading:1747074-Culture Media,
pubmed-meshheading:1747074-Dentin,
pubmed-meshheading:1747074-Epithelial Cells,
pubmed-meshheading:1747074-Epithelium,
pubmed-meshheading:1747074-Evaluation Studies as Topic,
pubmed-meshheading:1747074-Immunohistochemistry,
pubmed-meshheading:1747074-Incisor,
pubmed-meshheading:1747074-Phenotype,
pubmed-meshheading:1747074-Rats
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Morphological and immunocytochemical characterization of cultured rat incisor cervical epithelial cells.
|
| pubmed:affiliation |
Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, Faculté d'Odontologie, CNRS UPR 412, Lyon, France.
|
| pubmed:publicationType |
Journal Article
|