Source:http://linkedlifedata.com/resource/pubmed/id/17462502
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-4-27
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| pubmed:abstractText |
Renal transplant rejection and graft versus host reactions between HLA genetically-identical sibling (HLAgi) donor/recipient (D/R) pairs are thought to result from minor histocompatibility antigen (mHAg) disparities. We have compared two methods of measuring HLAgi D/R T lymphocyte responses to "matured" dendritic cells: 1.) a modified Cylex assay of CD4(+) ATP levels (MLDC-ATP) versus 2.) (3)H-thymidine uptake (MLDC-(3)H). The MLDC-ATP kinetics peaked at 48 hours versus the MLDC-(3)H at 7 days, and appeared more sensitive. We tested HLAgi (normal) volunteer siblings (NLs), and D/R sibling pairs before and after renal transplantation (pre-Tx and post-Tx). The overall frequencies of positive responses in the MLDC-ATP for HLAgi NLs, pre-Tx, and post-Tx D/R pairs were 63%, 50%, and 42%, respectively. The percentage with reciprocal responses was 37.5%, 20%, and 22.22%, respectively. In one set of three HLAgi (NLs) siblings (two males and one female), there was a nongender-associated differential response. There was no MLDC correlation with class I MHC-associated mHAg (SSP) incompatibility, nor could some MLDC positive reactive pairs theoretically process the necessary HLA-class I restriction molecules for presentation of known (nanomeric) mHAg peptides. Speculatively, the MLDC reflects class II MHC-restricted mHAg reactions (not yet definable), with possible effects of other polymorphic (nonhistocompatibility) immune response genes, and thereby may be a useful measurement of CD4(+) T-cell HLAgi transplantation immunity.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Minor Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0198-8859
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
68
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
350-61
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| pubmed:meshHeading |
pubmed-meshheading:17462502-Adenosine Triphosphate,
pubmed-meshheading:17462502-Autoantigens,
pubmed-meshheading:17462502-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17462502-Dendritic Cells,
pubmed-meshheading:17462502-Female,
pubmed-meshheading:17462502-HLA Antigens,
pubmed-meshheading:17462502-Histocompatibility,
pubmed-meshheading:17462502-Histocompatibility Antigens Class II,
pubmed-meshheading:17462502-Histocompatibility Testing,
pubmed-meshheading:17462502-Humans,
pubmed-meshheading:17462502-Kidney Transplantation,
pubmed-meshheading:17462502-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:17462502-Male,
pubmed-meshheading:17462502-Minor Histocompatibility Antigens,
pubmed-meshheading:17462502-Polymorphism, Genetic,
pubmed-meshheading:17462502-Sex Factors,
pubmed-meshheading:17462502-Siblings,
pubmed-meshheading:17462502-Thymidine,
pubmed-meshheading:17462502-Transplantation Immunology
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| pubmed:year |
2007
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| pubmed:articleTitle |
A novel approach to detect donor/recipent immune responses between HLA-identical pairs.
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| pubmed:affiliation |
Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation of the Department of Surgery, University of Miami, Leonard M. Miller School of Medicine, Miami, Florida 33101, USA. YJin@med.miami.edu
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| pubmed:publicationType |
Journal Article
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