Linked Life Data

17462108

Source:http://linkedlifedata.com/resource/pubmed/id/17462108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-4-27
pubmed:abstractText
It is often difficult to determine the effects of various substances on the development of the sea urchin embryo due to the lack of appropriate quantitative microassays. Here, a microplate assay has been developed for quantitatively evaluating the effects of substances, such as hyalin, on living sea urchin embryos. Hyalin (330 kDa) is a major constituent of the sea urchin hyaline layer, an extracellular matrix that develops 20 min postinsemination. Function of the hyaline layer and its major constituent, is the adhesion of cells during morphogenesis. Using wide-mouthed pipette tips, 25 microl of 24-h Strongylocentrotus purpuratus embryos were transferred to each well of a 96-well polystyrene flat-bottom microplate yielding about 12 embryos per well. Specific concentrations of purified hyalin diluted in low calcium seawater were added to the wells containing the embryos, which were then incubated for 24 h at 15 degree C. The hyalin-treated and control samples were observed live and after fixation with 10% formaldehyde using a Zeiss Axiolab photomicroscope. The small number of embryos in each well allowed quantification of the developmental effects of the added media. Specific archenteron morphologies-attached, unattached, no invagination and exogastrula-were scored and a dose-dependent response curve was generated. Hyalin at high concentrations blocked invagination. At low concentrations, it inhibited archenteron elongation/attachment to the blastocoel roof. While many studies have implicated hyalin in a variety of interactions during morphogenesis, we are not aware of any past studies that have quantitatively examined the effects of exogenous hyalin on specific gastrulation events in whole embryos.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-12781780, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-1371289, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-14099826, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-16261771, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-2424907, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-2578117, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-5118735, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-6180943, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-6661780, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-6998990, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-720411, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-720412, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-7635048, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-9138906, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-9473317, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-9587630, http://linkedlifedata.com/resource/pubmed/commentcorrection/17462108-9851856
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0967-1994
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-64
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Microplate assay for quantifying developmental morphologies: effects of exogenous hyalin on sea urchin gastrulation.
pubmed:affiliation
Department of Biology, California State University, Northridge, 18111 Nordhoff Street, Northridge, CA 91330-8303, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural