Source:http://linkedlifedata.com/resource/pubmed/id/17457364
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2007-11-16
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| pubmed:abstractText |
[(11)C]PK11195 is used in positron emission tomography (PET) studies for imaging brain inflammation in vivo as it binds to the peripheral-type benzodiazepine receptor (PBR) expressed by reactive glia and macrophages. However, features of the cellular reaction required to induce a positive [(11)C]PK11195 signal are not well characterized. We performed [(11)C]PK11195 PET and autoradiography in rats after transient focal cerebral ischemia. We determined [(3)H]PK11195 binding and PBR expression in brain tissue and examined the lesion with several markers. [(11)C]PK11195 standard uptake value increased at day 4 and grew further at day 7 within the ischemic core. Accordingly, ex vivo [(3)H]PK11195 binding increased at day 4, and increases further at day 7. The PET signal also augmented in peripheral regions, but to a lesser extent than in the core. Binding in the region surrounding infarction was supported by [(11)C]PK11195 autoradiography at day 7 showing that the radioactive signal extended beyond the infarcted core. Enhanced binding was preceded by increases in PBR mRNA expression in the ipsilateral hemisphere, and a 18-kDa band corresponding to PBR protein was detected. Peripheral-type benzodiazepine receptor immunohistochemistry showed subsets of ameboid microglia/macrophages within the infarcted core showing a distinctive strong PBR expression from day 4. These cells were often located surrounding microhemorrhages. Reactive astrocytes forming a rim surrounding infarction at day 7 also showed some PBR immunostaining. These results show cellular heterogeneity in the level of PBR expression, supporting that PBR is not a simple marker of inflammation, and that the extent of [(11)C]PK11195 binding depends on intrinsic features of the inflammatory cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/PK 11195,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Radiopharmaceuticals,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, GABA-A
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
0271-678X
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| pubmed:author |
pubmed-author:ArranzMaria JMJ,
pubmed-author:ChamorroAngelA,
pubmed-author:GómezVanessaV,
pubmed-author:GispertJoan DJD,
pubmed-author:LlopJordiJ,
pubmed-author:MartínAbrahamA,
pubmed-author:MillánOlgaO,
pubmed-author:ParetoDeborahD,
pubmed-author:PlanasAnna MAM,
pubmed-author:PurroyJesúsJ,
pubmed-author:RojasSantiagoS,
pubmed-author:VerdaguerEstherE
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| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1975-86
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| pubmed:meshHeading |
pubmed-meshheading:17457364-Animals,
pubmed-meshheading:17457364-Autoradiography,
pubmed-meshheading:17457364-Blotting, Western,
pubmed-meshheading:17457364-Brain,
pubmed-meshheading:17457364-Cerebral Hemorrhage,
pubmed-meshheading:17457364-Cerebral Infarction,
pubmed-meshheading:17457364-Image Processing, Computer-Assisted,
pubmed-meshheading:17457364-Immunohistochemistry,
pubmed-meshheading:17457364-Inflammation,
pubmed-meshheading:17457364-Ischemic Attack, Transient,
pubmed-meshheading:17457364-Isoquinolines,
pubmed-meshheading:17457364-Male,
pubmed-meshheading:17457364-Positron-Emission Tomography,
pubmed-meshheading:17457364-RNA, Messenger,
pubmed-meshheading:17457364-Radiopharmaceuticals,
pubmed-meshheading:17457364-Rats,
pubmed-meshheading:17457364-Rats, Sprague-Dawley,
pubmed-meshheading:17457364-Receptors, GABA-A,
pubmed-meshheading:17457364-Reperfusion Injury
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| pubmed:year |
2007
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| pubmed:articleTitle |
Imaging brain inflammation with [(11)C]PK11195 by PET and induction of the peripheral-type benzodiazepine receptor after transient focal ischemia in rats.
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| pubmed:affiliation |
Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomèdiques de Barcelona (IIBB)-Consejo Superior de Investigaciones Científicas (CSIC), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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