Source:http://linkedlifedata.com/resource/pubmed/id/17456199
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-4-25
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| pubmed:abstractText |
Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a pivotal role in the regulation of immune responses. Hence, we evaluated the effects of a recombinant adeno-associated viral vector 1 (rAAV1) encoding rat IL-10 (rAAV1-IL-10) in a rat model of kidney allograft rejection. Dark Agouti rat kidneys were transplanted into Wistar-Furth (WF) rats 8 weeks following a single intramuscular administration of either rAAV1-IL-10 or rAAV1-green fluorescence protein (GFP). Isografts (WF-WF) served as an additional experimental control. Both allograft and isograft recipients received daily cyclosporine (10 mg/kg) for 14 days after transplantation. Serum IL-10 levels increased at 8, 12 and 16 weeks following vector administration in rAAV1-IL-10-treated animals, but not in rAAV1-GFP and isograft groups. rAAV1-IL-10 treatment resulted in lower BUN and creatinine levels (p<0.001), as well as increased allograft survival rates from 22% to 90%. Allograft histological abnormalities were significantly attenuated in the rAAV1-IL-10-treated rats compared with those of rAAV1-GFP controls. Serum levels of proinflammatory cytokines such as growth-related oncogene were also significantly higher in the rAAV1-GFP group than in the rAAV1-IL-10 group. These data suggest delivery of IL-10 using a rAAV1 vector improves renal function and prolongs graft survival in a rat model of kidney transplant rejection.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1600-6135
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1112-20
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| pubmed:dateRevised |
2007-12-3
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| pubmed:meshHeading |
pubmed-meshheading:17456199-Animals,
pubmed-meshheading:17456199-Blood Urea Nitrogen,
pubmed-meshheading:17456199-Creatinine,
pubmed-meshheading:17456199-Cytokines,
pubmed-meshheading:17456199-Dependovirus,
pubmed-meshheading:17456199-Female,
pubmed-meshheading:17456199-Genetic Vectors,
pubmed-meshheading:17456199-Graft Rejection,
pubmed-meshheading:17456199-Graft Survival,
pubmed-meshheading:17456199-Green Fluorescent Proteins,
pubmed-meshheading:17456199-Injections, Intramuscular,
pubmed-meshheading:17456199-Interleukin-10,
pubmed-meshheading:17456199-Kidney,
pubmed-meshheading:17456199-Kidney Transplantation,
pubmed-meshheading:17456199-Models, Animal,
pubmed-meshheading:17456199-Rats,
pubmed-meshheading:17456199-Rats, Inbred Strains,
pubmed-meshheading:17456199-Rats, Inbred WF,
pubmed-meshheading:17456199-Transplantation, Homologous
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Adeno-associated viral vector-mediated interleukin-10 prolongs allograft survival in a rat kidney transplantation model.
|
| pubmed:affiliation |
Department of Medicine, Nephrology Research and Training Center, University of Alabama at Birmingham, Birmingham, Alabama, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|