Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-5-7
pubmed:abstractText
Culture of bovine embryos in the presence of insulin-like growth factor-1 (IGF-1) can increase pregnancy rates following transfer to heat-stressed, lactating dairy cows. The objective of the present experiment was to determine whether the effect of IGF-1 on post-transfer embryo survival was a general effect or one specific to heat stress. Lactating recipients (n=311) were synchronized for timed-embryo transfer at four locations. Embryos were produced in vitro and cultured with or without 100 ng/mL IGF-1. At Day 7 after anticipated ovulation (Day 0), a single embryo was randomly transferred to each recipient. Pregnancy was diagnosed at Day 21 by elevated plasma progesterone concentrations, at Days 27-32 by ultrasonography, and at Days 41-49 by transrectal palpation. Transfers were categorized into two seasons, hot or cool (based on the month of transfer). There was a tendency (P<0.09) for an interaction between embryo treatment and season for pregnancy rate at Day 21; this interaction was significant at Days 30 and 45 (P<0.02). Recipients receiving IGF-1 treated embryos had higher pregnancy rates in the hot season but not in the cool season. There was a similar interaction between embryo treatment and season for overall calving rate (P<0.05). There was also an interaction between season and treatment affecting pregnancy loss between Days 21 and 30; recipients that received IGF-1 treated embryos had less pregnancy loss during this time period in the hot season but not in the cool season. The overall proportion of male calves born was 77.5%. In conclusion, treatment of embryos with IGF-1 improved pregnancy and calving rates following the transfer of in vitro produced embryos into lactating recipients, but only under heat-stress conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0093-691X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1518-29
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:17452048-Abortion, Veterinary, pubmed-meshheading:17452048-Animals, pubmed-meshheading:17452048-Birth Weight, pubmed-meshheading:17452048-Cattle, pubmed-meshheading:17452048-Culture Media, pubmed-meshheading:17452048-Embryo, Mammalian, pubmed-meshheading:17452048-Embryo Culture Techniques, pubmed-meshheading:17452048-Embryo Transfer, pubmed-meshheading:17452048-Female, pubmed-meshheading:17452048-Fertilization in Vitro, pubmed-meshheading:17452048-Insulin-Like Growth Factor I, pubmed-meshheading:17452048-Lactation, pubmed-meshheading:17452048-Live Birth, pubmed-meshheading:17452048-Male, pubmed-meshheading:17452048-Pregnancy, pubmed-meshheading:17452048-Pregnancy Rate, pubmed-meshheading:17452048-Seasons, pubmed-meshheading:17452048-Sex Ratio, pubmed-meshheading:17452048-Temperature, pubmed-meshheading:17452048-Time Factors
pubmed:year
2007
pubmed:articleTitle
Interaction between season and culture with insulin-like growth factor-1 on survival of in vitro produced embryos following transfer to lactating dairy cows.
pubmed:affiliation
Department of Animal Sciences, University of Florida, Gainesville, FL 32611-0910, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't