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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-4-23
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pubmed:databankReference |
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pubmed:abstractText |
A new full-length cDNA (Ssd12) encoding a Delta12-fatty acid desaturase (Delta12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods. Ssd12 contained a 1146 bp open reading frame encoding a protein of 381 amino acids. The amino acid sequence showed a much higher match with microsomal Delta12-FAD amino acid sequences than chloroplast Delta12-FAD amino acid sequences. Genomic Southern blot analysis suggested that Ssd12 had at least two copies. Ssd12 transcripts were detected in roots, leaves, stems, and seeds by real time PCR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
959-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17450326-Amino Acid Sequence,
pubmed-meshheading:17450326-Blotting, Southern,
pubmed-meshheading:17450326-Cloning, Molecular,
pubmed-meshheading:17450326-Fatty Acid Desaturases,
pubmed-meshheading:17450326-Fatty Acids,
pubmed-meshheading:17450326-Gene Expression Profiling,
pubmed-meshheading:17450326-Genome, Plant,
pubmed-meshheading:17450326-Molecular Sequence Data,
pubmed-meshheading:17450326-Plant Leaves,
pubmed-meshheading:17450326-Plant Proteins,
pubmed-meshheading:17450326-Plant Roots,
pubmed-meshheading:17450326-Plant Stems,
pubmed-meshheading:17450326-Recombinant Proteins,
pubmed-meshheading:17450326-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17450326-Saccharomyces cerevisiae,
pubmed-meshheading:17450326-Sapium,
pubmed-meshheading:17450326-Seeds,
pubmed-meshheading:17450326-Sequence Homology, Amino Acid
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pubmed:year |
2007
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pubmed:articleTitle |
Cloning and characterization of a novel Delta12-fatty acid desaturase gene from the tree Sapium sebiferum.
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pubmed:affiliation |
College of Life Science, Sichuan University, Chengdu, China.
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pubmed:publicationType |
Journal Article
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