|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2007-5-2
|
|
pubmed:abstractText |
Chromosome segregation and proper alignment in mitosis relies on cohesion between sister chromatids and the interaction of the kinetochore with spindle microtubules. Vertebrate Sgo 1 localizes to kinetochores and is required to prevent premature sister centromere separation in mitosis. Sgo 1 is degraded by the anaphase-promoting complex, allowing the separation of sister centromeres in anaphase. However, little is known about the molecular basis of Sgo 1 degradation and its temporal control during mitosis. Here, we show that APC/C targets human Sgo 1 for degradation through a destruction box motif (D-box) in its C-terminus. Mutation in the D-box causes transient metaphase arrest, and mutation in the D-box leads to defects in chromosome alignment and segregation through its effect on the localization of Aurora B and CENP-E. These results provide a link between sister centromere cohesion and bipolar attachment of kinetochores.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
8
|
|
pubmed:volume |
357
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
672-8
|
|
pubmed:dateRevised |
2011-7-11
|
|
pubmed:meshHeading |
pubmed-meshheading:17448445-Amino Acid Sequence,
pubmed-meshheading:17448445-Anaphase,
pubmed-meshheading:17448445-Binding Sites,
pubmed-meshheading:17448445-Blotting, Western,
pubmed-meshheading:17448445-Cell Cycle,
pubmed-meshheading:17448445-Cell Cycle Proteins,
pubmed-meshheading:17448445-Cell Line,
pubmed-meshheading:17448445-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:17448445-Chromosome Segregation,
pubmed-meshheading:17448445-Green Fluorescent Proteins,
pubmed-meshheading:17448445-HeLa Cells,
pubmed-meshheading:17448445-Humans,
pubmed-meshheading:17448445-Microscopy, Confocal,
pubmed-meshheading:17448445-Mitosis,
pubmed-meshheading:17448445-Mutation,
pubmed-meshheading:17448445-Protein-Serine-Threonine Kinases,
pubmed-meshheading:17448445-Recombinant Fusion Proteins,
pubmed-meshheading:17448445-Time Factors,
pubmed-meshheading:17448445-Transfection
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
D-box is required for the degradation of human Shugoshin and chromosome alignment.
|
|
pubmed:affiliation |
Laboratory of Cellular Dynamics, University of Science & Technology of China, Hefei National Laboratory, Hefei 230027, China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
