Linked Life Data

17446433

Source:http://linkedlifedata.com/resource/pubmed/id/17446433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-5-14
pubmed:abstractText
Invasion of the subendothelial space by vascular smooth muscle cells (VSMCs) contributes to the development and progression of diverse cardiovascular diseases. In this report we show that the expression of activated versions of Src, Cdc42 and Rac1, or a kinase-dead but open form of the p21-activated kinase (PAK1), induces primary rat aorta VSMCs to form extracellular matrix-degrading actin-rich protrusions that are morphologically similar to the invadopodia formed by highly invasive tumor cells. The matrix-degrading structures are enriched in known markers for invadopodia, including cortactin and tyrosine-phosphorylated cortactin and contain the matrix metalloproteinases MMP-9 and MT1-MMP and the urokinase plasminogen activator receptor (uPAR). In contrast to other cell types, invadopodia formation in VSMCs is only weakly supported by the phorbol ester PBDu. Invadopodia formation by Src was dependent on Cdc42, Rac, and ERK, but not on p38 MAPK. Invadopodia formation induced by kinase-dead PAK1 required Src and ERK activity and a direct interaction with the exchange factor PIX. VSMCs embedded in a three-dimensional collagen matrix formed actin- and cortactin-rich extensions that penetrated through holes in the matrix, suggesting that invadopodia-like structures are formed in a three-dimensional environment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1328-36
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17446433-Animals, pubmed-meshheading:17446433-Aorta, pubmed-meshheading:17446433-Cell Movement, pubmed-meshheading:17446433-Cell Surface Extensions, pubmed-meshheading:17446433-Cells, Cultured, pubmed-meshheading:17446433-Collagen, pubmed-meshheading:17446433-Extracellular Matrix, pubmed-meshheading:17446433-Microscopy, Fluorescence, pubmed-meshheading:17446433-Muscle, Smooth, Vascular, pubmed-meshheading:17446433-Myocytes, Smooth Muscle, pubmed-meshheading:17446433-Phorbol 12,13-Dibutyrate, pubmed-meshheading:17446433-Protein-Serine-Threonine Kinases, pubmed-meshheading:17446433-Rats, pubmed-meshheading:17446433-cdc42 GTP-Binding Protein, pubmed-meshheading:17446433-p21-Activated Kinases, pubmed-meshheading:17446433-rac GTP-Binding Proteins, pubmed-meshheading:17446433-src-Family Kinases
pubmed:year
2007
pubmed:articleTitle
Formation of extracellular matrix-digesting invadopodia by primary aortic smooth muscle cells.
pubmed:affiliation
Department of Biochemistry, Queen's University, Kingston, ON, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't