Linked Life Data

17445853

Source:http://linkedlifedata.com/resource/pubmed/id/17445853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-5-14
pubmed:abstractText
The cytochrome P4501C (CYP1C) gene subfamily was recently discovered in fish, and zebrafish (Danio rerio) CYP1C1 transcript has been cloned. Here we cloned the paralogous CYP1C2, showing that the amino acid sequence is 78% identical to CYP1C1, and examined gene structure and expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2. Xenobiotic response elements were observed upstream of the coding regions in all four genes. Zebrafish adults and embryos were exposed (24 h) to 100 nM 3,3',4,4',5-polychlorinated biphenyl (PCB126) or 20 ppm acetone and subsequently held in clean water for 24 h (adults) or 48 h (embryos). All adult organs examined (eye, gill, heart, liver, kidney, brain, gut, and gonads) and embryos showed basal expression of the four genes. CYP1A was most strongly expressed in liver, whereas CYP1B1, CYP1C1, and CYP1C2 were most strongly expressed in heart and eye. CYP1B1 and the CYP1C genes showed an expression pattern similar to one another and to mammalian CYP1B1. In embryos CYP1C1 and CYP1C2 tended to have a higher basal expression than CYP1A and CYP1B1. PCB126 induced CYP1A in all organs, and CYP1B1 and CYP1C1 in all organs except gonads, or gonads and brain, respectively. CYP1C2 induction was significant only in the liver. However, in embryos all four genes were induced strongly by PCB126. The results are consistent with CYP1C1 and CYP1C2, as well as CYP1A and CYP1B1, being regulated by the aryl hydrocarbon receptor. While CYP1A may have a protective role against AHR agonists in liver and gut, CYP1B1, CYP1C1, and CYP1C2 may also play endogenous roles in eye and heart and possibly other organs, as well as during development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
221
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-41
pubmed:dateRevised
2011-1-17
pubmed:meshHeading
pubmed-meshheading:17445853-Amino Acid Sequence, pubmed-meshheading:17445853-Animals, pubmed-meshheading:17445853-Aryl Hydrocarbon Hydroxylases, pubmed-meshheading:17445853-Cloning, Molecular, pubmed-meshheading:17445853-Cytochrome P-450 CYP1A1, pubmed-meshheading:17445853-Cytochrome P-450 Enzyme System, pubmed-meshheading:17445853-Dose-Response Relationship, Drug, pubmed-meshheading:17445853-Embryo, Nonmammalian, pubmed-meshheading:17445853-Estrogen Antagonists, pubmed-meshheading:17445853-Female, pubmed-meshheading:17445853-Gene Expression Profiling, pubmed-meshheading:17445853-Gene Expression Regulation, Developmental, pubmed-meshheading:17445853-Gene Expression Regulation, Enzymologic, pubmed-meshheading:17445853-Isoenzymes, pubmed-meshheading:17445853-Male, pubmed-meshheading:17445853-Molecular Sequence Data, pubmed-meshheading:17445853-Polychlorinated Biphenyls, pubmed-meshheading:17445853-Sequence Homology, Amino Acid, pubmed-meshheading:17445853-Zebrafish, pubmed-meshheading:17445853-Zebrafish Proteins, pubmed-meshheading:17445853-beta-Naphthoflavone
pubmed:year
2007
pubmed:articleTitle
Basal and 3,3',4,4',5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish.
pubmed:affiliation
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA. mjonsson@whoi.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural