Source:http://linkedlifedata.com/resource/pubmed/id/17445852
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2007-5-14
|
| pubmed:abstractText |
Toxicity of the polychlorinated biphenyls (PCBs) depends on their molecular structure. Mechanisms by prenatal exposure to a non-dioxin-like PCB, 2,2',3,4',5',6-hexachlorobiphenyl (PCB 132) that may act on reproductive pathways in male offspring are relatively unknown. The purpose was to determine whether epididymal sperm function and expression of apoptosis-related genes were induced or inhibited by prenatal exposure to PCB 132. Pregnant rats were treated with a single dose of PCB 132 at 1 or 10 mg/kg on gestational day 15. Male offspring were killed and the epididymal sperm counts, motility, velocity, reactive oxygen species (ROS) generation, sperm-oocyte penetration rate (SOPR), testicular histopathology, apoptosis-related gene expression and caspase activation were assessed on postnatal day 84. Prenatal exposure to PCB 132 with a single dose of 1 or 10 mg/kg decreased cauda epididymal weight, epididymal sperm count and motile epididymal sperm count in adult offspring. The spermatozoa of PCB 132-exposed offspring produced significantly higher levels of ROS than the controls; ROS induction and SOPR reduction were dose-related. In the low-dose PCB 132 group, p53 was significantly induced and caspase-3 was inhibited. In the high-dose group, activation of caspase-3 and -9 was significantly increased, while the expressions of Fas, Bax, bcl-2, and p53 genes were significantly decreased. Gene expression and caspase activation data may provide insight into the mechanisms by which exposure to low-dose or high-dose PCB 132 affects reproduction in male offspring in rats. Because the doses of PCB 132 administered to the dams were approximately 625-fold in low-dose group and 6250-fold higher in high-dose group than the concentration in human tissue levels, the concentrations are not biologically or environmentally relevant. Further studies using environmentally relevant doses are needed for hazard identification.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2',3,3',4,6'-hexachlorobiphenyl,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Polychlorinated Biphenyls,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf6 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0041-008X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
221
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
68-75
|
| pubmed:meshHeading |
pubmed-meshheading:17445852-Animals,
pubmed-meshheading:17445852-Animals, Newborn,
pubmed-meshheading:17445852-Antigens, CD95,
pubmed-meshheading:17445852-Apoptosis,
pubmed-meshheading:17445852-Caspase 3,
pubmed-meshheading:17445852-Caspase 9,
pubmed-meshheading:17445852-Dose-Response Relationship, Drug,
pubmed-meshheading:17445852-Enzyme Activation,
pubmed-meshheading:17445852-Female,
pubmed-meshheading:17445852-Gene Expression,
pubmed-meshheading:17445852-Injections, Intraperitoneal,
pubmed-meshheading:17445852-Male,
pubmed-meshheading:17445852-Models, Biological,
pubmed-meshheading:17445852-Polychlorinated Biphenyls,
pubmed-meshheading:17445852-Pregnancy,
pubmed-meshheading:17445852-Prenatal Exposure Delayed Effects,
pubmed-meshheading:17445852-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:17445852-Rats,
pubmed-meshheading:17445852-Rats, Sprague-Dawley,
pubmed-meshheading:17445852-Reactive Oxygen Species,
pubmed-meshheading:17445852-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17445852-Spermatozoa,
pubmed-meshheading:17445852-Testis,
pubmed-meshheading:17445852-Tumor Suppressor Protein p53,
pubmed-meshheading:17445852-bcl-2-Associated X Protein
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Exposure in utero to 2,2',3,3',4,6'-hexachlorobiphenyl (PCB 132) impairs sperm function and alters testicular apoptosis-related gene expression in rat offspring.
|
| pubmed:affiliation |
Department of Safety, Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, Kaohsiung, Taiwan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|