Linked Life Data

17443768

Source:http://linkedlifedata.com/resource/pubmed/id/17443768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-4-19
pubmed:abstractText
Inductive signals mediating the differentiation of neural precursors into serotonergic (5-HT) or dopaminergic neurons have not been clarified. We have recently shown that in cell aggregates obtained from rat mesencephalic precursors, reduction of serotonin levels induces a marked increase in generation of dopaminergic neurons. In the present study we treated rat neurospheres with antagonists of the main subtypes of 5-HT receptors, 5-HT transport inhibitors, or 5-HT receptor agonists, and studied the effects on generation of dopaminergic neurons. Cultures treated with Methiothepin (5-HT(1,2,5,6,7) receptor antagonist), the 5-HT(4) receptor antagonist GR113808;67:00-.or the 5-HT(7) receptor antagonist SB 269970 showed a significant increase in generation of dopaminergic cells. Treatment with the 5-HT(1B/1D) antagonist GR 127935, the 5-HT(2) antagonist Ritanserin, the 5-HT transporter inhibitor Fluoxetine, the dopamine and norepinephrine transport inhibitor GBR 12935, or with both inhibitors together, or 5-HT(4) or 5-HT(7) receptor agonists induced significant decreases in generation of dopaminergic cells. Cultures treated with WAY100635 (5-HT(1A) receptor antagonist), the 5-HT(3) receptor antagonist Ondasetron, or the 5-HT(6) receptor antagonist SB 258585 did not show any significant changes. Therefore, 5-HT(4) and 5-HT(7) receptors are involved in the observed serotonin-induced decrease in generation of dopaminergic neurons from proliferating neurospheres of mesencephalic precursors. 5-HT(4) and 5-HT(7) receptors were found in astrocytes and serotonergic cells using double immunolabeling and laser confocal microscopy, and the glial receptors appeared to play a major role.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1932-8451
pubmed:author
pubmed:copyrightInfo
(c) 2006 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-22
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:17443768-Animals, pubmed-meshheading:17443768-Cell Differentiation, pubmed-meshheading:17443768-Cells, Cultured, pubmed-meshheading:17443768-Dopamine, pubmed-meshheading:17443768-Dose-Response Relationship, Drug, pubmed-meshheading:17443768-Embryo, Mammalian, pubmed-meshheading:17443768-Fluoxetine, pubmed-meshheading:17443768-Mesencephalon, pubmed-meshheading:17443768-Neurons, pubmed-meshheading:17443768-Neurotransmitter Uptake Inhibitors, pubmed-meshheading:17443768-Rats, pubmed-meshheading:17443768-Rats, Sprague-Dawley, pubmed-meshheading:17443768-Receptors, Serotonin, pubmed-meshheading:17443768-Receptors, Serotonin, 5-HT4, pubmed-meshheading:17443768-Serotonin, pubmed-meshheading:17443768-Serotonin Antagonists, pubmed-meshheading:17443768-Stem Cells, pubmed-meshheading:17443768-Tyrosine 3-Monooxygenase
pubmed:year
2007
pubmed:articleTitle
Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors.
pubmed:affiliation
Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't