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rdf:type |
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lifeskim:mentions |
umls-concept:C0016030,
umls-concept:C0022262,
umls-concept:C0030233,
umls-concept:C0037813,
umls-concept:C0086418,
umls-concept:C0205245,
umls-concept:C0205314,
umls-concept:C0368608,
umls-concept:C0439810,
umls-concept:C0456603,
umls-concept:C0521115,
umls-concept:C0599748,
umls-concept:C0679622,
umls-concept:C0680730,
umls-concept:C1123023,
umls-concept:C1148554,
umls-concept:C1158368,
umls-concept:C1510438,
umls-concept:C2003903,
umls-concept:C2348693
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pubmed:issue |
1-2
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pubmed:dateCreated |
2007-5-28
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pubmed:abstractText |
Two separate and complementary assays, total mitochondrial fatty acid beta-oxidation (FAO) flux rate and acylcarnitine profiling, have been used to establish a definitive diagnosis of FAO defects (FAOD) in cultured cells. We developed a novel functional assay for total FAO rate assay by measurement of deuterated water enrichment and to combine it with the conventional acylcarnitine profiling method into a single tracer incubation experiment.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-8981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
382
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-30
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pubmed:meshHeading |
pubmed-meshheading:17442290-Carnitine,
pubmed-meshheading:17442290-Cell Line,
pubmed-meshheading:17442290-Deuterium,
pubmed-meshheading:17442290-Fatty Acids,
pubmed-meshheading:17442290-Fibroblasts,
pubmed-meshheading:17442290-Humans,
pubmed-meshheading:17442290-Lipid Metabolism, Inborn Errors,
pubmed-meshheading:17442290-Mitochondria,
pubmed-meshheading:17442290-Multienzyme Complexes,
pubmed-meshheading:17442290-Oxidation-Reduction,
pubmed-meshheading:17442290-Palmitates,
pubmed-meshheading:17442290-Skin,
pubmed-meshheading:17442290-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:17442290-Tandem Mass Spectrometry
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pubmed:year |
2007
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pubmed:articleTitle |
A novel functional assay for simultaneous determination of total fatty acid beta-oxidation flux and acylcarnitine profiling in human skin fibroblasts using (2)H(31)-palmitate by isotope ratio mass spectrometry and electrospray tandem mass spectrometry.
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pubmed:affiliation |
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT Hong Kong SAR.
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pubmed:publicationType |
Journal Article,
Validation Studies
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