Linked Life Data

1744041

Source:http://linkedlifedata.com/resource/pubmed/id/1744041

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1992-1-15
pubmed:databankReference
pubmed:abstractText
A strain of Bacillus species which produced an enzyme named glutaryl 7-ACA acylase which converts 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl 7-ACA) to 7-amino cephalosporanic acid (7-ACA) was isolated from soil. The gene for the glutaryl 7-ACA acylase was cloned with pHSG298 in Escherichia coli JM109, and the nucleotide sequence was determined by the M13 dideoxy chain termination method. The DNA sequence revealed only one large open reading frame composed of 1,902 bp corresponding to 634 amino acid residues. The deduced amino acid sequence contained a potential signal sequence in its amino-terminal region. Expression of the gene for glutaryl 7-ACA acylase was performed in both E. coli and Bacillus subtilis. The enzyme preparations purified from either recombinant strain of E. coli or B. subtilis were shown to be identical with each other as regards the profile of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were composed of a single peptide with the molecular size of 70 kDa. Determination of the amino-terminal sequence of the two enzyme preparations revealed that both amino-terminal sequences (the first nine amino acids) were identical and completely coincided with residues 28 to 36 of the open reading frame. Extracellular excretion of the enzyme was observed in a recombinant strain of B. subtilis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-2824449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-2993240, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-2993245, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-3108260, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-3145387, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-3214149, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-3327753, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-3527045, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-4894690, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-6263856, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-6438629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-806078, http://linkedlifedata.com/resource/pubmed/commentcorrection/1744041-809282
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7848-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Cloning and nucleotide sequencing of a novel 7 beta-(4-carboxybutanamido)cephalosporanic acid acylase gene of Bacillus laterosporus and its expression in Escherichia coli and Bacillus subtilis.
pubmed:affiliation
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Ibaraki, Japan.
pubmed:publicationType
Journal Article