Linked Life Data

17439414

Source:http://linkedlifedata.com/resource/pubmed/id/17439414

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2007-4-18
pubmed:abstractText
1. The aim of the present study was to investigate the changes in chemotherapeutic drug sensitivity of HepG2 cells transfected with Bcl-2 and Bcl-xl siRNA expression vectors. 2. Bcl-2 and Bcl-xl siRNA and negative siRNA expression vectors were constructed and stably transfected into HepG2 cells. Reverse transcriptase-polymerase chain reaction was used to detect the target gene expression, and the Bcl-2, Bcl-xl, Bax and caspase-3 protein levels were measured using western blots and immunofluorescence. The sensitivity of the cells to the chemotherapeutic drugs 5-fluorouracil (5-FU) and 10-hydroxycamptothecin (HCPT) was analysed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) and flow cytometry. 3. The Bcl-2 and Bcl-xl gene expression and corresponding protein levels in Bcl-2 siRNA, Bcl-xl siRNA and Bcl-2/Bcl-xl siRNA transfected cells were reduced compared with negative siRNA transfected or untreated cells. The Bax protein level remained unaltered but the caspase-3 level was enhanced when Bcl-2 and Bcl-xl protein levels were reduced. The MTT results demonstrated that Bcl-2 and Bcl-xl transfected cells exhibited increased sensitivity to 5-FU or HCPT. Flow cytometry demonstrated that the sub G1 cell population increased in Bcl-2/Bcl-xl siRNA co-transfected and Bcl-xl siRNA and Bcl-2 siRNA transfected cells when compared with negative siRNA or untreated cells. The latter trend was strengthened further in the presence of 5-FU or HCPT. 4. Thus, Bcl-2 and Bcl-xl siRNA-mediated gene silencing, in combination with chemotherapy, may be a potential therapeutic strategy against human hepatoblastoma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0305-1870
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
450-6
pubmed:meshHeading
pubmed-meshheading:17439414-Antineoplastic Agents, pubmed-meshheading:17439414-Apoptosis, pubmed-meshheading:17439414-Camptothecin, pubmed-meshheading:17439414-Caspase 3, pubmed-meshheading:17439414-Cell Line, Tumor, pubmed-meshheading:17439414-Cell Survival, pubmed-meshheading:17439414-Down-Regulation, pubmed-meshheading:17439414-Drug Resistance, Neoplasm, pubmed-meshheading:17439414-Flow Cytometry, pubmed-meshheading:17439414-Fluorouracil, pubmed-meshheading:17439414-Gene Expression Regulation, Neoplastic, pubmed-meshheading:17439414-Gene Silencing, pubmed-meshheading:17439414-Humans, pubmed-meshheading:17439414-Microscopy, Fluorescence, pubmed-meshheading:17439414-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:17439414-RNA, Messenger, pubmed-meshheading:17439414-RNA, Small Interfering, pubmed-meshheading:17439414-Time Factors, pubmed-meshheading:17439414-Transfection, pubmed-meshheading:17439414-bcl-X Protein
pubmed:articleTitle
siRNA-mediated Bcl-2 and Bcl-xl gene silencing sensitizes human hepatoblastoma cells to chemotherapeutic drugs.
pubmed:affiliation
Institute of Pharmacy and Pharmacology, University of South China, Hengyang, Hunan, China. lei_xiaoyong@yahoo.com.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't