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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-4-17
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pubmed:abstractText |
Transplantation of isolated hepatocytes has been proposed to compensate for essential functions lacking in liver failure or for genetic defects that alter a specific liver metabolic pathway. Hepatocyte utilization for these purposes would be facilitated with a reliable, reproducible, and effective method of long-term hepatocyte storage. We have recently developed a simple new system for cryopreservation of hepatocytes that encapsulates alginate microspheres and maintains liver-specific function. The aim of this study was to elucidate the transport and drug-metabolizing enzyme activities of cryopreserved microencapsulated hepatocytes stored for a long time. Morphological examinations showed there is no apparent injury of the hepatocytes during cryopreservation processes. A drug-metabolizing enzyme (testosterone 6beta-hydroxylase, a specific probe for CYP3A2) and drug transport activities [salicylate, allopurinol, and prostaglandin E2 (PGE2), typical substrates of rOat2] in cryopreserved microencapsulated hepatocytes were maintained up to 120 days. Our results thus demonstrate for the first time that cryopreservation of primary rat hepatocytes by the encapsulation technique allows long-term retention of drug metabolism and drug transport activities.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0963-6897
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pubmed:author |
pubmed-author:AokiTakeshiT,
pubmed-author:EnamiYutaY,
pubmed-author:IzumidaYoshihikoY,
pubmed-author:JinZhenghaoZ,
pubmed-author:KatoHirohisaH,
pubmed-author:KobayashiYasunaY,
pubmed-author:KoizumiTomotakeT,
pubmed-author:KusanoMitsuoM,
pubmed-author:MitamuraKeitaroK,
pubmed-author:MuraiNoriyukiN,
pubmed-author:NiiyaTakashiT,
pubmed-author:NishinoNobukazuN,
pubmed-author:ShimizuYoshinoriY,
pubmed-author:YamamotoToshinoriT,
pubmed-author:YasudaDaisukeD
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
67-73
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pubmed:meshHeading |
pubmed-meshheading:17436856-Allopurinol,
pubmed-meshheading:17436856-Animals,
pubmed-meshheading:17436856-Biological Transport,
pubmed-meshheading:17436856-Cell Transplantation,
pubmed-meshheading:17436856-Cryopreservation,
pubmed-meshheading:17436856-Cytochrome P-450 Enzyme System,
pubmed-meshheading:17436856-Dinoprostone,
pubmed-meshheading:17436856-Hepatocytes,
pubmed-meshheading:17436856-Male,
pubmed-meshheading:17436856-Microsomes,
pubmed-meshheading:17436856-Rats,
pubmed-meshheading:17436856-Rats, Sprague-Dawley,
pubmed-meshheading:17436856-Salicylates,
pubmed-meshheading:17436856-Steroid Hydroxylases,
pubmed-meshheading:17436856-Time Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Long-term maintenance of the drug transport activity in cryopreservation of microencapsulated rat hepatocytes.
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pubmed:affiliation |
Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. t-koiz@med.showa-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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