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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2007-5-7
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pubmed:databankReference |
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pubmed:abstractText |
Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastomaxglioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
395
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
144-50
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17433858-Amino Acid Sequence,
pubmed-meshheading:17433858-Animals,
pubmed-meshheading:17433858-Base Sequence,
pubmed-meshheading:17433858-Brain,
pubmed-meshheading:17433858-Cell Line,
pubmed-meshheading:17433858-Cloning, Molecular,
pubmed-meshheading:17433858-DNA, Complementary,
pubmed-meshheading:17433858-DNA Primers,
pubmed-meshheading:17433858-Expressed Sequence Tags,
pubmed-meshheading:17433858-Gene Expression Profiling,
pubmed-meshheading:17433858-Gene Expression Regulation, Developmental,
pubmed-meshheading:17433858-Humans,
pubmed-meshheading:17433858-In Situ Hybridization,
pubmed-meshheading:17433858-Mice,
pubmed-meshheading:17433858-Mice, Inbred ICR,
pubmed-meshheading:17433858-Molecular Sequence Data,
pubmed-meshheading:17433858-Nerve Tissue Proteins,
pubmed-meshheading:17433858-Open Reading Frames,
pubmed-meshheading:17433858-Promoter Regions, Genetic,
pubmed-meshheading:17433858-Rats,
pubmed-meshheading:17433858-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17433858-Sequence Homology, Amino Acid,
pubmed-meshheading:17433858-Transfection
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pubmed:year |
2007
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pubmed:articleTitle |
Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display.
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pubmed:affiliation |
Department of Life Science, Dongguk University, Seoul 100-715, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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