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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-6-5
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pubmed:abstractText |
PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-10645449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-10694978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-10815743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-11069256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-12089271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-12654653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-12870683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-14532194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-14532345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-1501713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-15478353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-15675557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-15732733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-15793126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-15910223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16390942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16455882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16466036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16499254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16547071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16672384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16825345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16842572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16845631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-16870753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-8878604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-9041419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-9076716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-9210694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428939-9650946
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1789-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17428939-Antitubercular Agents,
pubmed-meshheading:17428939-Bacterial Proteins,
pubmed-meshheading:17428939-Catalase,
pubmed-meshheading:17428939-Drug Resistance, Bacterial,
pubmed-meshheading:17428939-Humans,
pubmed-meshheading:17428939-Isoniazid,
pubmed-meshheading:17428939-Microbial Sensitivity Tests,
pubmed-meshheading:17428939-Mutation,
pubmed-meshheading:17428939-Mycobacterium tuberculosis,
pubmed-meshheading:17428939-Polymerase Chain Reaction,
pubmed-meshheading:17428939-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:17428939-Predictive Value of Tests,
pubmed-meshheading:17428939-Sensitivity and Specificity,
pubmed-meshheading:17428939-Time Factors,
pubmed-meshheading:17428939-Tuberculosis, Pulmonary
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pubmed:year |
2007
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pubmed:articleTitle |
PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis.
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pubmed:affiliation |
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Vietnam. mcaws@hotmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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