| pubmed-article:17428902 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0229216 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0035298 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0022009 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:17428902 | lifeskim:mentions | umls-concept:C0599668 | lld:lifeskim |
| pubmed-article:17428902 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:17428902 | pubmed:dateCreated | 2007-6-7 | lld:pubmed |
| pubmed-article:17428902 | pubmed:abstractText | Recent studies have shown that cholinergic amacrine cells possess unique membrane properties. However, voltage-gated ionic channels in cholinergic amacrine cells have not been characterized systematically. In this study, using electrophysiological and immunohistochemical techniques, we examined voltage-gated ionic channels in a transgenic mouse line the cholinergic amacrine cells of which were selectively labeled with green fluorescent protein (GFP). Voltage-gated K(+) currents contained a 4-aminopyridine-sensitive current (A current) and a tetraethylammonium-sensitive current (delayed rectifier K(+) current). Voltage-gated Ca(2+) currents contained a omega-conotoxin GVIA-sensitive component (N-type) and a omega-Aga IVA-sensitive component (P/Q-type). Tetrodotoxin-sensitive Na(+) currents and dihydropyridine-sensitive Ca(2+) currents (L-type) were not observed. Immunoreactivity for the Na channel subunit (Pan Nav), the K channel subunits (the A-current subunits [Kv. 3.3 and Kv 3.4]) and the Ca channel subunits (alpha1(A) [P/Q-type], alpha1(B) [N-type] and alpha1(C) [L-type]) was detected in the membrane fraction of the mouse retina by Western blot analysis. Immunoreactivity for the Kv. 3.3, Kv 3.4, alpha1(A) [P/Q-type], and alpha1(B) [N-type] was colocalized with the GFP signals. Immunoreactivity for alpha1(C) [L-type] was not colocalized with the GFP signals. Immunoreactivity for Pan Nav did not exist on the membrane surface of the GFP-positive cells. Our findings indicate that signal propagation in cholinergic amacrine cells is mediated by a combination of two types of voltage-gated K(+) currents (the A current and the delayed rectifier K(+) current) and two types of voltage-gated Ca(2+) currents (the P/Q-type and the N-type) in the mouse retina. | lld:pubmed |
| pubmed-article:17428902 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17428902 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428902 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17428902 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:17428902 | pubmed:issn | 0022-3077 | lld:pubmed |
| pubmed-article:17428902 | pubmed:author | pubmed-author:ItoKoichiK | lld:pubmed |
| pubmed-article:17428902 | pubmed:author | pubmed-author:KanedaMakotoM | lld:pubmed |
| pubmed-article:17428902 | pubmed:author | pubmed-author:MorishimaYosu... | lld:pubmed |
| pubmed-article:17428902 | pubmed:author | pubmed-author:ShigematsuYas... | lld:pubmed |
| pubmed-article:17428902 | pubmed:author | pubmed-author:ShimodaYukioY | lld:pubmed |
| pubmed-article:17428902 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17428902 | pubmed:volume | 97 | lld:pubmed |
| pubmed-article:17428902 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17428902 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17428902 | pubmed:pagination | 4225-34 | lld:pubmed |
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| pubmed-article:17428902 | pubmed:meshHeading | pubmed-meshheading:17428902... | lld:pubmed |
| pubmed-article:17428902 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17428902 | pubmed:articleTitle | Characterization of voltage-gated ionic channels in cholinergic amacrine cells in the mouse retina. | lld:pubmed |
| pubmed-article:17428902 | pubmed:affiliation | Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan. mkaneda@sc.itc.keio.ac.jp | lld:pubmed |
| pubmed-article:17428902 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17428902 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17428902 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17428902 | lld:pubmed |
