Source:http://linkedlifedata.com/resource/pubmed/id/17428902
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2007-6-7
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| pubmed:abstractText |
Recent studies have shown that cholinergic amacrine cells possess unique membrane properties. However, voltage-gated ionic channels in cholinergic amacrine cells have not been characterized systematically. In this study, using electrophysiological and immunohistochemical techniques, we examined voltage-gated ionic channels in a transgenic mouse line the cholinergic amacrine cells of which were selectively labeled with green fluorescent protein (GFP). Voltage-gated K(+) currents contained a 4-aminopyridine-sensitive current (A current) and a tetraethylammonium-sensitive current (delayed rectifier K(+) current). Voltage-gated Ca(2+) currents contained a omega-conotoxin GVIA-sensitive component (N-type) and a omega-Aga IVA-sensitive component (P/Q-type). Tetrodotoxin-sensitive Na(+) currents and dihydropyridine-sensitive Ca(2+) currents (L-type) were not observed. Immunoreactivity for the Na channel subunit (Pan Nav), the K channel subunits (the A-current subunits [Kv. 3.3 and Kv 3.4]) and the Ca channel subunits (alpha1(A) [P/Q-type], alpha1(B) [N-type] and alpha1(C) [L-type]) was detected in the membrane fraction of the mouse retina by Western blot analysis. Immunoreactivity for the Kv. 3.3, Kv 3.4, alpha1(A) [P/Q-type], and alpha1(B) [N-type] was colocalized with the GFP signals. Immunoreactivity for alpha1(C) [L-type] was not colocalized with the GFP signals. Immunoreactivity for Pan Nav did not exist on the membrane surface of the GFP-positive cells. Our findings indicate that signal propagation in cholinergic amacrine cells is mediated by a combination of two types of voltage-gated K(+) currents (the A current and the delayed rectifier K(+) current) and two types of voltage-gated Ca(2+) currents (the P/Q-type and the N-type) in the mouse retina.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Voltage-Dependent Anion Channels
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0022-3077
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
97
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4225-34
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| pubmed:meshHeading |
pubmed-meshheading:17428902-Acetylcholine,
pubmed-meshheading:17428902-Amacrine Cells,
pubmed-meshheading:17428902-Animals,
pubmed-meshheading:17428902-Blotting, Western,
pubmed-meshheading:17428902-Calcium Channel Blockers,
pubmed-meshheading:17428902-Dose-Response Relationship, Drug,
pubmed-meshheading:17428902-Electric Stimulation,
pubmed-meshheading:17428902-Green Fluorescent Proteins,
pubmed-meshheading:17428902-Immunohistochemistry,
pubmed-meshheading:17428902-Ion Channel Gating,
pubmed-meshheading:17428902-Mice,
pubmed-meshheading:17428902-Mice, Inbred C57BL,
pubmed-meshheading:17428902-Mice, Transgenic,
pubmed-meshheading:17428902-Patch-Clamp Techniques,
pubmed-meshheading:17428902-Potassium Channel Blockers,
pubmed-meshheading:17428902-Retina,
pubmed-meshheading:17428902-Sodium Channel Blockers,
pubmed-meshheading:17428902-Voltage-Dependent Anion Channels
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| pubmed:year |
2007
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| pubmed:articleTitle |
Characterization of voltage-gated ionic channels in cholinergic amacrine cells in the mouse retina.
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| pubmed:affiliation |
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan. mkaneda@sc.itc.keio.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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