Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-5-28
pubmed:abstractText
The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. In this study, we show that the overexpression of Bcl-XL, a prosurvival member of the Bcl-2 family, blocks 7a-induced apoptosis, suggesting that the mechanism for apoptosis induction by 7a is at the level of or upstream from the Bcl-2 family. Coimmunoprecipitation experiments showed that 7a interacts with Bcl-XL and other prosurvival proteins (Bcl-2, Bcl-w, Mcl-1, and A1) but not with the proapoptotic proteins (Bax, Bak, Bad, and Bid). A good correlation between the abilities of 7a deletion mutants to induce apoptosis and to interact with Bcl-XL was observed, suggesting that 7a triggers apoptosis by interfering directly with the prosurvival function of Bcl-XL. Interestingly, amino acids 224 and 225 within the C-terminal transmembrane domain of Bcl-XL are essential for the interaction with the 7a protein, although the BH3 domain of Bcl-XL also contributes to this interaction. In addition, fractionation experiments showed that 7a colocalized with Bcl-XL at the endoplasmic reticulum as well as the mitochondria, suggesting that they may form complexes in different membranous compartments.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-12368257, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-12493639, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-12655348, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-12845623, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-12972498, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14592761, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14660106, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14681510, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14736283, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14767982, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14996494, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-14996505, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15131699, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15170624, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15194747, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15220404, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15294014, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15539520, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15564512, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15686503, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-15958670, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16048439, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16101347, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16107218, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16155806, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16182592, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16212942, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16282490, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16310778, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16378980, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16378996, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16494729, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16553810, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16676007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16759866, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16820226, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16845612, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16965829, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-16996569, http://linkedlifedata.com/resource/pubmed/commentcorrection/17428862-9927423
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6346-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.
pubmed:affiliation
Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Republic of Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't