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rdf:type |
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lifeskim:mentions |
umls-concept:C0007765,
umls-concept:C0010837,
umls-concept:C0027882,
umls-concept:C0032821,
umls-concept:C0162508,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0871712,
umls-concept:C1332838,
umls-concept:C1333925,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
42
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pubmed:dateCreated |
2007-10-15
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pubmed:abstractText |
In cerebellar granule neurons, a BH3-only Bcl-2 family member, death protein 5/harakiri, is up-regulated in a JNK-dependent manner during apoptosis induced by potassium deprivation. However, it is not clear whether c-Jun is directly involved in the induction of dp5. Here, we showed that the up-regulation of dp5, but not fas ligand and bim, after potassium deprivation was suppressed by the expression of a dominant negative form of c-Jun. Deletion analysis of the 5'-flanking sequence of the dp5 gene revealed that a major responsive element responsible for the induction by potassium deprivation is an ATF binding site located at -116 to -109 relative to the transcriptional start site. Mutation of this site completely abolished promoter activation. Furthermore, a gel shift assay showed that a specific complex containing c-Jun and ATF2 recognized this site and increased in potassium-deprived cerebellar granule neurons. Chromatin immunoprecipitation demonstrated that c-Jun was able to bind to this site in vivo. Finally, we demonstrated that knockdown of Dp5 by small interfering RNA rescued neurons from potassium deprivation-induced apoptosis. Taken together, these results suggest that dp5 is a target gene of c-Jun and plays a critical role in potassium deprivation-induced apoptosis in cerebellar granule neurons.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Atf2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Bcl-2-like protein 11,
http://linkedlifedata.com/resource/pubmed/chemical/Bid3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:AliNN,
pubmed-author:ChenRuzhuR,
pubmed-author:ChingYick-PangYP,
pubmed-author:LaiBingquanB,
pubmed-author:LiDanD,
pubmed-author:LiMingtaoM,
pubmed-author:LiWenmingW,
pubmed-author:LiuYulinY,
pubmed-author:SongBinB,
pubmed-author:YingChunyiC,
pubmed-author:YuanZhongminZ
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30901-9
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pubmed:meshHeading |
pubmed-meshheading:17428807-Activating Transcription Factor 2,
pubmed-meshheading:17428807-Animals,
pubmed-meshheading:17428807-Apoptosis,
pubmed-meshheading:17428807-Apoptosis Regulatory Proteins,
pubmed-meshheading:17428807-Base Sequence,
pubmed-meshheading:17428807-Cells, Cultured,
pubmed-meshheading:17428807-Cerebellum,
pubmed-meshheading:17428807-Cytoplasmic Granules,
pubmed-meshheading:17428807-Fas Ligand Protein,
pubmed-meshheading:17428807-Genes, Dominant,
pubmed-meshheading:17428807-Genes, jun,
pubmed-meshheading:17428807-Membrane Proteins,
pubmed-meshheading:17428807-Neurons,
pubmed-meshheading:17428807-Neuropeptides,
pubmed-meshheading:17428807-Potassium,
pubmed-meshheading:17428807-Proto-Oncogene Proteins,
pubmed-meshheading:17428807-Rats,
pubmed-meshheading:17428807-Rats, Sprague-Dawley,
pubmed-meshheading:17428807-Response Elements,
pubmed-meshheading:17428807-Sequence Deletion,
pubmed-meshheading:17428807-Transcription, Genetic,
pubmed-meshheading:17428807-Up-Regulation
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pubmed:year |
2007
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pubmed:articleTitle |
dp5/HRK is a c-Jun target gene and required for apoptosis induced by potassium deprivation in cerebellar granule neurons.
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pubmed:affiliation |
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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