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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2007-4-18
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pubmed:abstractText |
Mobile DNAs use many mechanisms to minimize damage to their hosts. Here we show that a subclass of group II introns avoids host damage by inserting directly after transcriptional terminator motifs in bacterial genomes (stem-loops followed by Ts). This property contrasts with the site-specific behavior of most group II introns, which insert into homing site sequences. Reconstituted ribonucleo protein particles of the Bacillus halodurans intron B.h.I1 are shown to reverse-splice into DNA targets in vitro but require the DNA to be single-stranded and fold into a stem-loop analogous to the RNA structure that forms during transcription termination. Recognition of this DNA stem-loop motif accounts for in vivo target specificity. Insertion after terminators is a previously unrecognized strategy for a selfish DNA because it prevents interruption of coding sequences and restricts expression of the mobile DNA after integration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-10356323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-10541559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-10716944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-10760141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11222775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11274116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11371159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11497432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11713323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-11861899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12453213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12455970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12559910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12662921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12667460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12777534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12888493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12897009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-12941706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-14567911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-14616071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-15568970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-15813740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-16093660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-16230535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-16341091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-16641988,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-7585955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-7679954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-7878467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-9242914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-9427403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-9657139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17420455-9737919
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6620-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2007
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pubmed:articleTitle |
Insertion of group II intron retroelements after intrinsic transcriptional terminators.
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pubmed:affiliation |
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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