Linked Life Data

17409499

Source:http://linkedlifedata.com/resource/pubmed/id/17409499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-4-5
pubmed:abstractText
1H-NMR spectroscopy of urine has been proved to be a powerful tool in metabonomic investigations in the field of chemical toxicity evaluation and disease diagnosis. However, most studies on urinary metabolite profiling by 1H-NMR have been conducted using rat urine. In the course of experiment on 1H-NMR analysis of urine samples of mice administered selenium compound, we noticed a substantial variation of chemical shift of citrate among individual mice. To clarify the effect of urinary pH on chemical shift of citrate, we compared 1H-NMR spectra of urine samples obtained from selenium-treated mice and untreated mice as well as those from untreated rats. The results clearly showed a linear relationship between urinary pH and chemical shift of citrate in 1H-NMR spectra both in mice and rats. The urine of mice exhibited a wider variation of pH, resulting in a wider variation of chemical shift of citrate than that of rats. We also recognized a clear peak of trimethylamine in urine of mice, but not that of rats. These data indicate that more attention should be paid to the characteristics of mouse urine with special reference to pH and trimethylamine metabolism in the analysis of NMR spectroscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0918-6158
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
667-70
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Effect of pH on 1H-NMR spectroscopy of mouse urine.
pubmed:affiliation
Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
pubmed:publicationType
Journal Article, Comparative Study