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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2007-4-18
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pubmed:abstractText |
Recognition of lysine-type peptidoglycan by peptidoglycan recognition protein (PGRP)-SA provokes the activation of the Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment of lysine-type peptidoglycan, a long glycan chain with short stem peptides, is a potent activator of the Drosophila Toll pathway and the prophenoloxidase activation cascade in the beetle Tenebrio molitor. Using this peptidoglycan fragment, we present biochemical evidence that clustering of PGRP-SA molecules on the peptidoglycan is required for the activation of the prophenoloxidase cascade. We subsequently highlight that the lysozyme-mediated partial digestion of highly cross-linked lysine-type peptidoglycan dramatically increases the binding of PGRP-SA, presumably by inducing clustering of PGRP-SA, which then recruits the Gram-negative bacteria-binding protein 1 homologue and a modular serine protease containing low-density lipoprotein and complement control protein domains. The crucial role of lysozyme in the prophenoloxidase activation cascade is further confirmed in vivo by using a lysozyme inhibitor. Taken together, we propose a model whereby lysozyme presents a processed form of lysine-type peptidoglycan for clustering of PGRP-SA that recruits Gram-negative bacteria-binding protein 1 and the modular serine protease, which leads to the activation of both the Toll and prophenoloxidase pathways.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-10827089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-110434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-11742401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-11872802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-11912488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-12692550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-12845326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-12923175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-1332865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-14582617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-14684822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-14722090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-14728663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15190055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15199957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15199959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15361936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15448690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15572450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15653304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-15791270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16006509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16362048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16421099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16428381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16461344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-16556841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-17024181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-7822328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-8159165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-9158729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-9523106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17409189-9685723
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:HaNam-ChulNC,
pubmed-author:JeByung-RokBR,
pubmed-author:KimChan-HeeCH,
pubmed-author:KimJung-HyunJH,
pubmed-author:KimSu-JinSJ,
pubmed-author:LeeBok-LuelBL,
pubmed-author:LeeHyeon-HwaHH,
pubmed-author:LeeWon-JaeWJ,
pubmed-author:LimJae-HongJH,
pubmed-author:OhByung-HaBH,
pubmed-author:ParkJi-WonJW,
pubmed-author:RohKyung-BaegKB,
pubmed-author:RyuJi-HwanJH
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6602-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2007
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pubmed:articleTitle |
Clustering of peptidoglycan recognition protein-SA is required for sensing lysine-type peptidoglycan in insects.
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pubmed:affiliation |
National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Busan 609-735, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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