Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2007-5-7
pubmed:abstractText
The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications in both traditional molecular biology and the emerging field of synthetic biology. However, the current cost of de novo synthesis-driven largely by reagent and handling costs-is a significant barrier to the widespread availability of such technology. In this work, we demonstrate, to our knowledge, the first gene synthesis in a microfluidic environment. The use of microfluidic technology greatly reduces reaction volumes and the corresponding reagent and handling costs. Additionally, microfluidic technology enables large numbers of complex reactions to be performed in parallel. Here, we report the fabrication of a multi-chamber microfluidic device and its use in carrying out the syntheses of several DNA constructs. Genes up to 1 kb in length were synthesized in parallel at minute starting oligonucleotide concentrations (10-25 nM) in four 500 nl reactors. Such volumes are one to two orders of magnitude lower than those utilized in conventional gene synthesis. The identity of all target genes was verified by sequencing, and the resultant error rate was determined to be 1 per 560 bases.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-10591650, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-10659856, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-10753110, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-11289415, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-12000848, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-12114528, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-12421762, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-12778056, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-14657399, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-14674446, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15307448, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15448182, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15477391, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15489334, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15496466, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15561997, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15616567, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15782200, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15800206, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-15800209, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-16175253, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-3472723, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-3863253, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-7590320, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-8173510, http://linkedlifedata.com/resource/pubmed/commentcorrection/17405768-8628665
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Parallel gene synthesis in a microfluidic device.
pubmed:affiliation
Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Evaluation Studies