Linked Life Data

1740475

Source:http://linkedlifedata.com/resource/pubmed/id/1740475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-3-26
pubmed:abstractText
At maturity, the spores of Dictyostelium are suspended in a viscous fluid droplet, with each spore being surrounded by its own spore coat. Certain glycoproteins characteristic of the spore coat are also dissolved in this fluid matrix after the spore coat is formed. To determine whether any proteins of the coat reside in this fluid phase earlier during the process of spore coat assembly, pairs of strains which differed in a spore coat protein carbohydrate marker were mixed and allowed to form spore coats in each other's presence. We reasoned that proteins belonging to an early, soluble, extracellular pool would be incorporated into the spore coats of both strains. To detect trans-incorporation, spores were labeled with a fluorescent antibody against the carbohydrate marker and each spore's fluorescence was analyzed by flow cytometry. Several proteins of both the outer and inner protein layers of the coat appeared to be faithfully and reciprocally trans-incorporated and hence judged to belong to a soluble, assembly-phase pool. Western blot analysis of sorted spores, and EM localization, confirmed this conclusion. In contrast, one outer-layer protein was not trans-incorporated, and was concluded to be insoluble at the time of secretion. Three classes of spore coat proteins can be described: (a) Insoluble from the time of secretion; (b) present in the early, soluble pool but not the late pool after spore coat formation; and (c) present in the soluble pool throughout spore coat assembly. These classes may, respectively: (a) Nucleate spore coat assembly; (b) comprise a scaffold defining the dimensions of the nascent spore coat; and (c) complete the assembly process by intercalation into the scaffold.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-1034498, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-1692841, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-1977501, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2096020, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2163344, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2471657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2587278, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2601718, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2612369, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-2726746, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-3370674, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-561442, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-6352373, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-6383202, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-6627403, http://linkedlifedata.com/resource/pubmed/commentcorrection/1740475-7024457
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1291-300
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Incorporation of protein into spore coats is not cell autonomous in Dictyostelium.
pubmed:affiliation
Department of Anatomy, College of Medicine, University of Florida, Gainesville 32610-0235.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't