Source:http://linkedlifedata.com/resource/pubmed/id/17403771
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2007-7-2
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pubmed:abstractText |
IL-12 and IL-18 synergistically induce the production of IFN-gamma by resting and activated T cells. To evaluate whether this induction was affected in HIV-1-infected patients, PBMC or isolated CD4 T cells were cultured with IL-12 plus IL-18, anti-CD3 plus anti-CD28, or PHA for 72 h. Cell samples were labeled daily to assess the levels of IL-12 receptor beta1 (IL-12Rbeta1), IL-12Rbeta2, and IL-18Ralpha. Culture supernatants were analyzed for the presence of Th1- and Th2-related cytokines by ELISA or cytometric bead array and analyzed by flow cytometry. A twofold increase in the percentage of CD4-resting T cells expressing IL-12Rbeta1 and IL-18Ralpha from HIV-1-infected patients was observed when compared with cells from HIV-1-negative donors. Higher IL-12Rbeta1 and IL-18Ralpha expression correlated (r=0.87; P<0.007) to increased production of IFN-gamma by isolated CD4 T cells in the presence of IL-12 and IL-18. Moreover, exogenous IL-12 and IL-18 induced the up-regulation of IL-12Rbeta2 to twice higher in CD4 T cells from HIV-1-positive individuals compared with controls. Conversely, upon activation with anti-CD3 and anti-CD28 antibodies, only 25% of the CD4+ T cells from HIV-1 patients showed an increase in the IL-12beta2 when compared with 50% in healthy controls. Furthermore, the percentage of IL-12Rbeta1-positive cells correlated inversely with the CD4 nadir of patients, suggesting that deregulation of the IL-12 and IL-18 pathways may play a role in the immunopathogenesis of HIV-1 infection.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0741-5400
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pubmed:author |
pubmed-author:BofillMargaritaM,
pubmed-author:CabreraCeciliaC,
pubmed-author:CalleM LuzML,
pubmed-author:ClotetBonaventutaB,
pubmed-author:ComaGemmaG,
pubmed-author:EstéJosé AJA,
pubmed-author:GuerolaRosaR,
pubmed-author:PeñaRuthR,
pubmed-author:Ruiz-HernandezRaulR,
pubmed-author:RuizLidiaL,
pubmed-author:de ArquerGuillermo RobertGR
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
72-8
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pubmed:meshHeading |
pubmed-meshheading:17403771-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17403771-Case-Control Studies,
pubmed-meshheading:17403771-Cells, Cultured,
pubmed-meshheading:17403771-Gene Expression Regulation,
pubmed-meshheading:17403771-HIV Infections,
pubmed-meshheading:17403771-HIV-1,
pubmed-meshheading:17403771-Humans,
pubmed-meshheading:17403771-Lymphocyte Activation,
pubmed-meshheading:17403771-Receptors, Interleukin-12,
pubmed-meshheading:17403771-Receptors, Interleukin-18,
pubmed-meshheading:17403771-Up-Regulation
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pubmed:year |
2007
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pubmed:articleTitle |
Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients.
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pubmed:affiliation |
Fundació IrsiCaixa, HIVACAT, Hospital Universitari Germans Trias i Pujol, Ctra Canyet sn, 08916 Badalona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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