17400406

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0036025, umls-concept:C0205245, umls-concept:C0205474, umls-concept:C0439855, umls-concept:C0449432, umls-concept:C1179435, umls-concept:C1524073, umls-concept:C1548799, umls-concept:C1704675, umls-concept:C1705248, umls-concept:C2603343
pubmed:issue	1-2
pubmed:dateCreated	2007-5-7
pubmed:abstractText	HTL1, a small gene of Saccharomyces cerevisiae, encodes a 78-aminoacid peptide that influences the performance of a wide range of cellular processes [Lanzuolo, C., Ederle, S., Pollice, A., Russo, F., Storlazzi, A., Pulitzer, J.F., 2001. The HTL1 gene,YCR020W-b of Saccharomyces cerevisiae is necessary for growth at 37 degrees C, and for the conservation of chromosome stability and fertility. Yeast, 18, 1317-1330]. Genetic interactions and co-immunoprecipitation experiments indicate a role for Htl1p in functions controlled by RSC, a multiprotein, ATP-dependent, chromatin-remodeling complex [Lu, Y.M., Lin, Y.R., Tsai, A., Hsao, Y.S., Li, C.C., Cheng, M.Y., 2003. Dissecting the pet18 mutation in Saccharomyces cerevisiae: HTL1 encodes a 7-kDa polypeptide that interacts with components of the RSC complex. Mol. Genet. Genomics., 269, 321-330] [Romeo, M.J., Angus-Hill, M.L., Sobering, A.K., Kamada, Y., Cairns, B.R., Levin, D.E., 2002. HTL1 encodes a novel factor that interacts with the Rsc chromatin-remodeling complex in Saccharomyces cerevisiae. Mol. Cell. Biol., 22, 8165-8174]. Htl1p and RSC components, share the property of associating with TBP a component of general multiprotein transcription factor TFIID [Sanders, S.L., Jennings, J., Canutescu, A., Link, A.J., Weil, P.A., 2002. Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol. Cell. Biol. 22, 4723-4738]. We confirm, by integrating genetic and biochemical experiments, that Htl1p binding to the RSC complex is direct and physiologically relevant and show that it is mediated by Rsc8p, a core component of the RSC complex. Deletion of HTL1, like depletion of RSC core subunits [Moreira, J.M., Holmberg, S., 1999. Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex Rsc. Embo J., 18, 2836-2844], leads to constitutive transcription of the CHA1 locus. This transcriptional phenotype exhibits variable penetrance. Deletion of HTL1 also leads to hydroxyurea hypersensitivity at 30 degrees C, suggesting a defect in replication/repair. This defect leads, during cell growth, to selection of mutations at the SIR3 locus that suppress hydroxyurea sensitivity.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7706761
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Formamides, http://linkedlifedata.com/resource/pubmed/chemical/HTL1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyurea, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RSC complex, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SIR3 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Silent Information Regulator..., http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/formamide
pubmed:status	MEDLINE
pubmed:month	Jun
pubmed:issn	0378-1119
pubmed:author	pubmed-author:EderleSaraS, pubmed-author:FasanoRossellaR, pubmed-author:FlorioCarolinaC, pubmed-author:LanzuoloChiaraC, pubmed-author:MoscarielloMarioM, pubmed-author:PulitzerJohn FJF
pubmed:issnType	Print
pubmed:day	15
pubmed:volume	395
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	72-85
pubmed:dateRevised	2009-7-23
pubmed:meshHeading	pubmed-meshheading:17400406-Amino Acid Sequence, pubmed-meshheading:17400406-Base Sequence, pubmed-meshheading:17400406-Chromatin Assembly and Disassembly, pubmed-meshheading:17400406-DNA, Fungal, pubmed-meshheading:17400406-DNA-Binding Proteins, pubmed-meshheading:17400406-Drug Resistance, Fungal, pubmed-meshheading:17400406-Escherichia coli, pubmed-meshheading:17400406-Formamides, pubmed-meshheading:17400406-Genes, Fungal, pubmed-meshheading:17400406-Hydroxyurea, pubmed-meshheading:17400406-Molecular Sequence Data, pubmed-meshheading:17400406-Mutation, pubmed-meshheading:17400406-Nuclear Proteins, pubmed-meshheading:17400406-Plasmids, pubmed-meshheading:17400406-Recombinant Proteins, pubmed-meshheading:17400406-Saccharomyces cerevisiae, pubmed-meshheading:17400406-Saccharomyces cerevisiae Proteins, pubmed-meshheading:17400406-Sequence Homology, Amino Acid, pubmed-meshheading:17400406-Silent Information Regulator Proteins, Saccharomyces..., pubmed-meshheading:17400406-Transcription Factors
pubmed:year	2007
pubmed:articleTitle	A study of biochemical and functional interactions of Htl1p, a putative component of the Saccharomyces cerevisiae, Rsc chromatin-remodeling complex.
pubmed:affiliation	Università degli Studi di Napoli Federico II, Dipartimento di Biologia, Via Cinthia-80126-Naples, Italy.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't