Source:http://linkedlifedata.com/resource/pubmed/id/17395646
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2007-4-16
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pubmed:abstractText |
GATA-2, a transcription factor that has been shown to play important roles in multiple organ systems during embryogenesis, has been ascribed the property of regulating the expression of numerous endothelium-specific genes. However, the transcriptional regulatory hierarchy governing Gata2 activation in endothelial cells has not been fully explored. Here, we document GATA-2 endothelial expression during embryogenesis by following GFP expression in Gata2-GFP knock-in embryos. Using founder transgenic analyses, we identified a Gata2 endothelium enhancer in the fourth intron and found that Gata2 regulation by this enhancer is restricted to the endocardial, lymphatic and vascular endothelium. Whereas disruption of three ETS-binding motifs within the enhancer diminished its activity, the ablation of its single E box extinguished endothelial enhancer-directed expression in transgenic mice. Development of the endothelium is known to require SCL (TAL1), and an SCL-E12 (SCL-Tcfe2a) heterodimer can bind the crucial E box in the enhancer in vitro. Thus, GATA-2 is expressed early in lymphatic, cardiac and blood vascular endothelial cells, and the pan-endothelium-specific expression of Gata2 is controlled by a discrete intronic enhancer.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0950-1991
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pubmed:author |
pubmed-author:BrandtWilliamW,
pubmed-author:DagenaisSusanS,
pubmed-author:EngelJames DouglasJD,
pubmed-author:GloverThomas WTW,
pubmed-author:KhandekarMelinM,
pubmed-author:LimKim-ChewKC,
pubmed-author:ShimizuRitsukoR,
pubmed-author:SuzukiNorioN,
pubmed-author:YamamotoMasayukiM,
pubmed-author:ZhouYinghuiY
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pubmed:issnType |
Print
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1703-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17395646-Animals,
pubmed-meshheading:17395646-Base Sequence,
pubmed-meshheading:17395646-Chromosomes, Artificial, Yeast,
pubmed-meshheading:17395646-Endothelium,
pubmed-meshheading:17395646-Enhancer Elements, Genetic,
pubmed-meshheading:17395646-GATA2 Transcription Factor,
pubmed-meshheading:17395646-Gene Expression Regulation,
pubmed-meshheading:17395646-Introns,
pubmed-meshheading:17395646-Mice,
pubmed-meshheading:17395646-Mice, Inbred Strains,
pubmed-meshheading:17395646-Mice, Transgenic,
pubmed-meshheading:17395646-Molecular Sequence Data
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pubmed:year |
2007
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pubmed:articleTitle |
A Gata2 intronic enhancer confers its pan-endothelia-specific regulation.
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pubmed:affiliation |
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109-2200, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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