Linked Life Data

17392278

Source:http://linkedlifedata.com/resource/pubmed/id/17392278

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2007-5-22
pubmed:abstractText
The p21-activated kinases (Paks) serve as effectors of the Rho family GTPases Rac and Cdc42. The six human Paks are divided into two groups based on sequence similarity. Group I Paks (Pak1 to -3) phosphorylate a number of substrates linking this group to regulation of the cytoskeleton and both proliferative and anti-apoptotic signaling. Group II Paks (Pak4 to -6) are thought to play distinct functional roles, yet their few known substrates are also targeted by Group I Paks. To determine if the two groups recognize distinct target sequences, we used a degenerate peptide library method to comprehensively characterize the consensus phosphorylation motifs of Group I and II Paks. We find that Pak1 and Pak2 exhibit virtually identical substrate specificity that is distinct from that of Pak4. Based on structural comparisons and mutagenesis, we identified two key amino acid residues that mediate the distinct specificities of Group I and II Paks and suggest a structural basis for these differences. These results implicate, for the first time, residues from the small lobe of a kinase in substrate selectivity. Finally, we utilized the Pak1 consensus motif to predict a novel Pak1 phosphorylation site in Pix (Pak-interactive exchange factor) and demonstrate that Pak1 phosphorylates this site both in vitro and in cultured cells. Collectively, these results elucidate the specificity of Pak kinases and illustrate a general method for the identification of novel sites phosphorylated by Paks.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15667-78
pubmed:dateRevised
2008-9-11
pubmed:meshHeading
pubmed-meshheading:17392278-Amino Acid Motifs, pubmed-meshheading:17392278-Cell Cycle Proteins, pubmed-meshheading:17392278-Cell Line, pubmed-meshheading:17392278-Guanine Nucleotide Exchange Factors, pubmed-meshheading:17392278-Humans, pubmed-meshheading:17392278-Mutagenesis, Site-Directed, pubmed-meshheading:17392278-Phosphorylation, pubmed-meshheading:17392278-Protein Processing, Post-Translational, pubmed-meshheading:17392278-Protein Structure, Tertiary, pubmed-meshheading:17392278-Protein-Serine-Threonine Kinases, pubmed-meshheading:17392278-Structural Homology, Protein, pubmed-meshheading:17392278-Structure-Activity Relationship, pubmed-meshheading:17392278-Substrate Specificity, pubmed-meshheading:17392278-cdc42 GTP-Binding Protein, pubmed-meshheading:17392278-rho GTP-Binding Proteins
pubmed:year
2007
pubmed:articleTitle
Specificity profiling of Pak kinases allows identification of novel phosphorylation sites.
pubmed:affiliation
Division of Basic Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural