Linked Life Data

17390664

Source:http://linkedlifedata.com/resource/pubmed/id/17390664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-3-29
pubmed:abstractText
The interaction of acridine orange (AO) and heparin was studied by spectrophotometry. In the pH 2. 0 Britton-Robinson (B-R) buffer solution, AO had a maximum absorption at 478 nm and 492 nm. After the addition of heparin, the absorbance value at 492 nm decreased greatly and a new absorption peak at 453 nm appeared, indicating a strong interaction taking place in the reaction solution. Under the optimal condition, the decrease in absorbance at 492 nm was linear with the concentration of heparin in the range of 3. 0-15.0 mg m L(-1). The m olar absorptivity w as calculated to be E=l. 599X 10(5) L mol(-1) x cm(-1) and the detection limit was 0. 236 mg x L(-1) (3sigma). The method was furthur applied to the detection of heparin sodium injection samples with satisfactory results. The binding number was calculated to be 1 : 3 with molar ratio method.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1000-0593
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
116-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[Spectrophotometric study on the interaction of acridine orange and heparin].
pubmed:affiliation
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. sunwei@qust.edu.cn
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't