Linked Life Data

17390067

Source:http://linkedlifedata.com/resource/pubmed/id/17390067

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-3-28
pubmed:abstractText
Human intersectins 1 and 2 (ITSN1 and ITSN2) are conserved proteins involved in clathrin-mediated endocytosis. In both, two major splice variants, the so-called long and short isoforms have been identified. Whereas most analyses so far focussed on ITSN1, little is known about ITSN2. Data from expression analyses for the intersectin genes mainly refer to the major isoforms. Only recently have a few minor splice variants of ITSN2 been described, though no detailed analyses of their expression in different tissues have been performed. Using RT-PCR-studies we analyzed ITSN2 minor splice variants and their expression in an adult tissue panel. Thereby we demonstrated at least one new minor variant lacking exon 7. Differential expression was demonstrated for a previously described minor splice variant including exon 16 (ITSN2C) with a relative increase in adult human brain tissue. Additional comparative expression analyses in oligodendrogliomas furthermore revealed differential expression with lack of this specific minor splice variant in the brain tumor tissue. These results indicate that ITSN2C may be specifically expressed in neurons hinting to a physiologic role in neuronal cell function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1207-11
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Expression analysis of human intersectin 2 gene (ITSN2) minor splice variants showing differential expression in normal human brain.
pubmed:affiliation
Department of Human Genetics, Saarland University Hospital, Homburg, Saar, Germany. hgmsei@uniklinik-saarland.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't