Source:http://linkedlifedata.com/resource/pubmed/id/17389738
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-4-26
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| pubmed:abstractText |
The inhibitory effects of recombinant human erythropoietin (rhEPO) were examined against (1) the progression of renal fibrosis in mice with complete unilateral ureteral obstruction and (2) the TGF-beta1-induced epithelial-to-mesenchymal transition (EMT) in MDCK cells. Unilateral ureteral obstruction was induced in BALB/c mice and rhEPO (100 or 1000 U/kg, intraperitoneally, every other day) or vehicle was administered from day 3 to day 14. Immunoblotting and immunohistochemistry revealed increased expressions of TGF-beta1, alpha-smooth muscle actin (alpha-SMA), and fibronectin and decreased expression of E-cadherin in the obstructed kidneys. In contrast, rhEPO treatment significantly attenuated the upregulation of TGF-beta1 and alpha-SMA and the downregulation of E-cadherin. MDCK cells were treated with TGF-beta1 (5 ng/ml) for 48 h to induce EMT, and the cells were then co-treated with TGF-beta1 and rhEPO for another 48 h. Increased expressions of alpha-SMA and vimentin and decreased expressions of zona occludens-1 and E-cadherin were observed after TGF-beta1 treatment, and these changes were markedly attenuated by rhEPO co-treatment. TGF-beta1 increased phosphorylated Smad-2 expression in MDCK cells, which was decreased by rhEPO co-treatment. In conclusion, rhEPO treatment inhibits the progression of renal fibrosis in obstructed kidney and attenuates the TGF-beta1-induced EMT. It is suggested that the renoprotective effects of rhEPO could be mediated, at least partly, by inhibition of TGF-beta1-induced EMT.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1046-6673
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| pubmed:author |
pubmed-author:ChoiMin-JeongMJ,
pubmed-author:ChoiSoon-YounSY,
pubmed-author:KimChan-DuckCD,
pubmed-author:KimIn-SanIS,
pubmed-author:KimYong-JinYJ,
pubmed-author:KimYong-LimYL,
pubmed-author:KwonTae-HwanTH,
pubmed-author:LeeByung-HeonBH,
pubmed-author:NamJu-OckJO,
pubmed-author:ParkKwon MooKM,
pubmed-author:ParkRang-WoonRW,
pubmed-author:ParkSun-HeeSH,
pubmed-author:SongIn-KyungIK
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| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1497-507
|
| pubmed:meshHeading |
pubmed-meshheading:17389738-Actins,
pubmed-meshheading:17389738-Animals,
pubmed-meshheading:17389738-Cells, Cultured,
pubmed-meshheading:17389738-Disease Progression,
pubmed-meshheading:17389738-Dogs,
pubmed-meshheading:17389738-Epithelial Cells,
pubmed-meshheading:17389738-Erythropoietin,
pubmed-meshheading:17389738-Fibronectins,
pubmed-meshheading:17389738-Fibrosis,
pubmed-meshheading:17389738-Humans,
pubmed-meshheading:17389738-Kidney,
pubmed-meshheading:17389738-Kidney Diseases,
pubmed-meshheading:17389738-Male,
pubmed-meshheading:17389738-Mesoderm,
pubmed-meshheading:17389738-Mice,
pubmed-meshheading:17389738-Mice, Inbred BALB C,
pubmed-meshheading:17389738-Recombinant Proteins,
pubmed-meshheading:17389738-Transforming Growth Factor beta1,
pubmed-meshheading:17389738-Ureteral Obstruction
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| pubmed:year |
2007
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| pubmed:articleTitle |
Erythropoietin decreases renal fibrosis in mice with ureteral obstruction: role of inhibiting TGF-beta-induced epithelial-to-mesenchymal transition.
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| pubmed:affiliation |
Division of Nephrology and Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Korea
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|